富自体浓缩生长因子膜在兔下颌骨牵张成骨中的作用  

Effect of concentrated growth factor fibrin membrane during distraction osteogenesis of the rabbit mandible

作　　者：田璐鸣[1] 辛增玺[1] 马云胜 

机构地区：[1]辽宁医学院附属第二医院口腔外科,辽宁省锦州市121001 [2]锦州医科大学发育生物学教研室,辽宁省锦州市121000

出　　处：《中国组织工程研究》2017年第8期1172-1177,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省教育厅基金项目(L2012308)~~

摘　　要：背景:富自体浓缩生长因子膜可参与调节代谢,已被用于骨缺损的重建。研究发现在牵张成骨的新骨缝附近的成骨细胞、间质细胞及骨细胞的胞质中均有骨保护素和核因子ΚB受体活化因子配体表达。目的:分析骨矫形作用的机制及富自体浓缩生长因子膜对兔下颌骨牵张成骨的促进意义。方法:随机选取24只大白兔建立兔下颌骨牵张成骨模型;对照组行左单侧下颌骨牵张成骨;实验组将富自体浓缩生长因子膜固定于牵张成骨器内侧面并且完全包绕牵张间隙,再行右单侧下颌骨牵张成骨;共延长6 mm。在固定期第1,7,14及28天分别处死动物,获取双侧下颌骨行组织学苏木精-伊红染色、免疫印迹法Western blot法和免疫组织化学检测核因子ΚB受体活化因子配体及骨保护素在新生骨中的表达情况;观察和对比两组间牵张间隙内成骨效果。结果与结论:牵引后第1,7,14天骨保护素表达的阳性细胞率和阳性面积百分比实验组均显著高于对照组(P<0.05或P<0.01);牵引后第1,14天核因子ΚB受体活化因子配体表达的阳性细胞率和阳性面积百分比实验组均显著高于对照组(P<0.05或P<0.01);Western blot法检测核因子ΚB受体活化因子配体/骨保护素比值对照组较实验组要高(P<0.01)。结果说明,富自体浓缩生长因子膜可促进兔下颌骨牵张成骨间隙内的新骨形成和矿化,说明富自体浓缩生长因子膜是促进下颌骨牵张成骨的有效手段。BACKGROUND： Concentrated growth factor （CGF） fibrin membrane is involved in metabolism regulation, which has been applied in bone remodeling. Previous Studies have found that the cytoplasts of osteoblasts, interstitial cells and osteocytes in distraction osteogenesis are positive for osteoprotegerin and receptor activator nuclear factor kappa B ligand （RANKL）.OBJECTIVE： To explore the mechanism of bone orthopedic effect and the promotion effect of CGF fibrin membrane on the distraction osteogenesis of the rabbit mandible.METHODS： Twenty-four white rabbits were randomly selected. Distraction osteogenesis was performed unilaterally in the rabbit left mandible （control group）; CGF fibrin membrane was fixed on the inner surface of distractor, and the distraction gap was then completely wrapped prior to distraction osteogenesis in the right mandible （experimental group）.At 1, 7, 14 and 28 days of consolidation period, the rabbits were sacrificed and the bilateral mandibles were taken for hematoxylin-eosin staining, western blot assay and immunohistochemical staining to detect the expression of RANKL and osteoprotegerin in the newborn bones; the osteogenesis in the distraction gap was observed and compared between two groups.RESULTS AND CONCLUSION： The osteoprotegerin positive cells and positive area percentage in the experimental group were significantly higher than those in the control group at 1, 7 and 14 days after distraction osteogenesis （P 〈 0.01）. The RANKL positive rate and positive area percentage in the experimental group were significantly higher than those in the control group at 1 and 14 days after distraction osteogenesis （P 〈 0.05 or P 〈 0.01）. Western blot assay showed a higher ratio of RANKL/osteoprotegerin in the control group than the experimental group （P 〈 0.01）. Our findings indicate that CGF fibrin membrane can promote osteogenesis and mineralization in the distraction gap of the rabbit mandible, which is beneficial for distraction osteogenesis in the

关 键 词：富血小板血浆 RANK配体 骨保护素 组织工程 组织构建 骨组织工程 富自体浓缩生长因子 RANKL 牵张成骨 免疫印迹法 

分 类 号：R318[医药卫生—生物医学工程]