热休克蛋白27在正畸牙张力侧牙周组织中的表达  

Expression of heat shock protein 27 in the periodontium on the tension side during orthodontic tooth movement

作　　者：聂晶[1] 李泽慧[2] 姜涵[2] 潘旭[1] 李春霞[1] 

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第一附属医院牙周粘膜科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2017年第8期1197-1201,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金青年项目(2014211C080);课题名称:热休克蛋白家族在正畸牙移动中的表达与调节机制的研究~~

摘　　要：背景:在正畸牙移动过程中,在力的作用下,牙周膜生成大量细胞因子,参与牙周组织的改建,使牙齿发生移动,牙槽骨重新塑形。热休克蛋白27就是众多参与牙周组织改建的细胞因子之一。目的:观察正畸牙移动过程大鼠张力侧牙周组织中热休克蛋白27的表达。方法:采用NiTi拉簧建立大鼠正畸牙移动模型,力值50 g。分别于加力后1,3,5,7,10,14 d取正畸牙周组织,检测热休克蛋白27在张力侧牙周组织中的表达。结果与结论:免疫荧光染色显示,正畸加力1 d,牙周组织中热休克蛋白70的表达量增强,至加力后3 d时表达量最大,之后逐渐降低,第5天时回落至初始水平。可以认为,在受到正畸力的初始阶段,即可刺激牙周组织中热休克蛋白27的表达,热休克蛋白27可能参与了张力侧牙周组织的改建。BACKGROUND： A large number of cytokines produce in the periodontium on the tension side during orthodontic tooth movement, participate in periodontal remodeling, and make the tooth move to its normal position, thus achieving alveolar remodeling. Heat shock protein 27 （HSP27） is reported to be involved in the periodontal remodeling. OBJECTIVE： To investigate the expression of HSP27 in the rat periodontium on the tension side during the orthodontic tooth movement.METHODS： The rat models of orthodontic tooth movement were constructed using NiTi coil spring with the force value of 50 g. The periodontal samples were collected at 1, 3, 5, 7, 10 and 14 days after exerting force to detect the expression level of HSP27 in the periodontium on the tension side.RESULTS AND CONCLUSION： Immunofluorescence staining results revealed that the expression level of HSP27 in the periodontal tissues began to increase at the 1st day after exerting force, reached the highest level at the 3rd day, and then fell to the initial level at the 5th day. To conclude, HSP27 is evoked by orthodontic force early, and it may play a role in periodontal remodeling.

关 键 词：热休克蛋白质类 牙 正畸学 组织工程 组织构建 骨组织工程 热休克蛋白 牙周组织 正畸牙移动 新疆维吾尔自治区自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]