聚(对氧环己酮-三亚甲基碳酸酯)静电纺膜的制备及其生物学与降解性能研究  

Preparation,Biological Properties and Degradation Studies on the Electrospun Membranes of Poly(p-dioxanone-co-trimethylene carbonate)

作　　者：李丽楠[1,2] 刘永江 刘书慧 杨欢[1,2] 熊成东[1] 陈栋梁[1] 

机构地区：[1]中国科学院成都有机化学研究所,成都610041 [2]中国科学院大学,北京100049 [3]青岛出入境检验检疫局,青岛266001

出　　处：《高分子学报》2017年第4期651-660,共10页Acta Polymerica Sinica

基　　金：中国科学院“西部之光”人才培养计划资助项目

摘　　要：通过静电纺丝技术,将二元共聚物聚(对氧环己酮-三亚甲基碳酸酯)(PDT)制成了膜材料,采用扫描电镜(SEM)观察了静电纺膜的形貌,当共聚物中TMC组分含量越高时,纤维丝之间黏结越严重.采用静态接触角仪测定了静电纺膜的接触角,表征了共聚物的亲疏水性,PDT的亲水性介于PPDO与PTMC均聚物静电纺膜之间.对PDT共聚物静电纺膜进行了L929大鼠成纤维细胞培养实验,MTT和荧光染色结果说明PDT膜的细胞相容性良好.通过水解及酶解实验考察了材料的降解过程,PDT静电纺膜在脂肪酶存在下降解速率加快,材料的失重率、分子量损失、材料形貌破坏程度等均高于水解样品,这说明脂肪酶对含有TMC组分的聚合物的降解有促进作用.同时,降解过程中PBS溶液的p H值变化说明TMC组分的加入,减缓了降解过程中酸性物质的产生.Nano-structured fibers of the synthesized poly（p-dioxanone-co-trimethylene carbonate） （PDT） were prepared by electrospinning technique, Their physicochemical and biological properties were evaluated with the standards for biomedical materials, and it was found that the content of trimethylene carbonate （TMC） units in PDT copolymer could change these properties. As observed by the scanning electron microscopy （SEM）, more TMC units in the copolymer would lead to severer fiber bonding, which probably is due to the ductility of TMC segments. The results of static water contact angle testing for the electrospun membranes demonstrated that the hydrophilicity of PDT was between that of PPDO and PTMC. The growth status of L929 mouse fibroblasts on the as-spun membranes of PDT and PPDO was evaluated by the fluorescent micrographs and MTT assay. It was shown that the cells grew well, which suggested good biocompatibility of these copolymers and their suitability in biomedical fields. The in vitro biodegradability of the electrospun membranes in phosphate buffered saline （PBS） and PBS with lipase was separately investigated via measurements of mass loss, Mw retention, as well as variations in crystallinity, thermal properties, pH value, and morphology. After 35 days of degradation, mass and Mw of the polymers significantly reduced while slight differences appeared in pH values before and after. Among the samples, the pH value exhibited the most descent for PBS with PPDO and the least for PBS with PTMC; pH decrement of PBS with PDT was in the middle. These results pointed out that the existence of TMC units in the polymers reduced acidic products in the degradation process, which was good for implanted materials. The changes of SEM images of PTMC at different degradation time indicated that PTMC degraded by surface erosion. And the changes of thermal properties of PDT indicated that PTMC was susceptible to enzymatic degradation by lipase. The lipase enzymatic degradation rate of the polymers containing TM

关 键 词：对氧环己酮 三亚甲基碳酸酯 静电纺膜 降解 

分 类 号：TQ340.64[化学工程—化纤工业] TB383.2[一般工业技术—材料科学与工程]