基于STM32的荧光侧流免疫层析试条定量检测仪  

A quantitative determination system for fluorescent lateral flow immunochromatographic strip based on STM32

作　　者：姜海燕[1,2] 黄弘舟 高跃明[3,2] 陈建国[1,2] JIANG Haiyan HUANG Hongzhou GAO Yueming CHEN Jianguo(a. School of Electrical Engineering and Automation 1 b. School of Physics and Information Engineering, Fuzhou University, Fuzhou 350116, Chin 2. Fujian Key Lab of Medical Instrumentation ＆ Pharmaceutical Technology, Fuzhou 350116, China)

机构地区：[1]福州大学电气工程与自动化学院,福建福州350116 [2]福建省医疗器械和医药技术重点实验室,福建福州350116 [3]福州大学物理与信息工程学院,福建福州350116

出　　处：《南昌大学学报（工科版）》2017年第1期88-91,共4页Journal of Nanchang University(Engineering & Technology)

基　　金：闽经信计财资助项目(2015567号)

摘　　要：介绍了以STM32为微控制器的荧光侧流免疫层析试条定量检测仪的设计,可实现对C-反应蛋白进行定量检测。检测仪由光学模块、光电传感器、荧光信号采集及调理电路、液晶显示及机械扫描装置等组成。通过对比试验表明:检测仪对C-反应蛋白标准溶液检测下限为1.95μg·m L^(-1),在1.95~256μg·m L^(-1)质量浓度范围内有良好的线性特性,检测仪的线性度和稳定性较好,可应用在生物医学、临床、环境检测等多个领域的快速检测。This paper introduced the design of quantitative detector for fluorescence lateral flow immunochromatographic strip with STM32 as a micro controller,which can realize the quantitative detection on C-reactive protein. The system was composed of a set of modules,including optical module,photoelectric sensor,and fluorescent signal acquisition,conditioning circuit,LCD display and mechanical scanning devices. The experimental test showed that the lower limit of detection（LOD） was 1. 95 μg·m L-1,and the linear range was 1. 95-256 μg·m L-1 for C-reactive protein（CRP） in the system. It has robust linearity and stability and can be utilized widely in different fields,such as biomedical detection,clinical application and environment determination.

关 键 词：荧光 测流免疫层析 定量检测 STM32 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]