姜黄素对阿尔茨海默病细胞模型的保护作用及GAP-43蛋白表达的影响  

Effects of curcumin on the cellular model of Alzheimer′s disease and GAP-43 expression

作　　者：张忠敏[1] 王晓莉[2] 毕鹏翔[1] 关亚新[1] 郭艳芹[1] 罗海龙[1] 

机构地区：[1]牡丹江医学院红旗医院神经内科,牡丹江157011 [2]牡丹江医学院红旗医院肾内科,牡丹江157011

出　　处：《国际药学研究杂志》2017年第3期257-261,共5页Journal of International Pharmaceutical Research

基　　金：黑龙江省卫生计生委科研课题(JS216H201)

摘　　要：目的观察姜黄素对阿尔茨海默病(AD)细胞模型的保护作用及其对生长相关蛋白-43(GAP-43)表达的影响。方法以Aβ25~35作用于原代培养大鼠海马神经元,建立AD细胞模型,通过噻唑蓝(MTT)法检测细胞存活率;实验分为空白对照组、模型组、10和20μmol/L姜黄素组,采用流式细胞术检测姜黄素对凋亡的影响;采用免疫细胞化学分析观察细胞突起生长情况,计算GAP-43阳性细胞率;采用Western印迹法检测GAP-43蛋白的表达。结果与模型组比较,姜黄素能显著减轻Aβ25~35的毒性损伤,神经元细胞存活率升高,凋亡率下降(P<0.01);可显著增加平均突起长度,增高GAP-43阳性细胞率和GAP-43表达(P<0.05或P<0.01)。结论姜黄素对AD细胞模型有保护作用,其机制可能与上调GAP-43的表达有关。Objective To investigate the protective effects of curcumin on the cellular model of Alzheimer＇s disease（AD） and the expression of growth associated protein-43（GAP-43）. Methods Aβ25~35 was used to treat the hippocampus neurons of rat and the cellular model of AD was established. The survival rate was detected by MTT assay. The cells were randomly divided into blank control,model,curcumin 10 and 20μmol/L groups. The effect of curcumin on apoptosis was assayed by flow cytometry. The pro？trusive length and GAP-43 positive cell rate were detected by immunocytochemistry. The expression of GAP-43 was detected by West？ern blot. Results Compared with the model group,curcumin significantly reduced the toxicity of Aβ25~35,increased the survival rate and decreased the apoptosis rate of the cells（P〈0.05）. It also significantly increased the average protrusive length,GAP-43 positive cell rate and GAP-43 expression（P〈0.05 or P〈0.01）. Conclusion The protective effect of curcumin on the cellular model of AD was likely related to the up-regulation of GAP-43 expression.

关 键 词：阿尔茨海默病 姜黄素 生长相关蛋白-43 

分 类 号：R285[医药卫生—中药学]