不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹特征相似性研究  

Study on the similarity of chemical fingerprints of different compatibility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis

作　　者：邹纯才[1] 徐启祥 鄢海燕[1] 朱磊[1] 章圣朋[1] 张叶明[1] 

机构地区：[1]皖南医学院药学院,芜湖241002

出　　处：《国际药学研究杂志》2017年第3期278-281,共4页Journal of International Pharmaceutical Research

基　　金：安徽高校省级自然科学研究重大项目(KJ2015ZD41;KJ2016SD60);安徽省科技攻关项目(12010402089)

摘　　要：目的研究不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹图谱的相似性,为瓜蒌皮与瓜蒌子的合理配伍比例提供理论依据。方法采用高效液相色谱法建立30∶70、35∶65、40∶60、45∶55、50∶50、55∶45共6种比例的瓜蒌皮-瓜蒌子及瓜蒌的化学指纹图谱及其共有模式,比较不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌化学指纹图谱共有模式间的相似度。结果上述6种比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹图谱共有模式间的相似度分别为0.8808±0.0407、0.8930±0.0236、0.8995±0.0195、0.9033±0.0190、0.9363±0.0082和0.8904±0.0144,其中瓜蒌皮-瓜蒌子比例为50∶50时相似度最高。各组与瓜蒌皮-瓜蒌子50∶50组比较,差异有统计学意义(P<0.05或0.01)。结论采用指纹图谱技术确定了瓜蒌皮-瓜蒌子的最佳配伍比例,为瓜蒌皮-瓜蒌子的合理配伍提供了理论依据。Objective To study the similarity of chemical fingerprints of different proportion of Gualoupi-Gualouzi and Fruc？tus Trichosanthis,so as to provide the theoretical basis for the reasonable proportion of Gualoupi-Gualouzi. Methods The HPLC fin？gerprints and the common patterns of 6 the proportion of the 6 kinds of compatibility（30：70,35：65,40：60,45：55,50：50 and 55：45）of Gualoupi-Gualouzi and Fructus Trichosanthis were established,and the similarity of chemical fingerprints of different compati？bility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis between their common patterns were compared. Results Compared with the common pattern of Fructus Trichosanthis,the similarities of the common patterns of chemical fingerprints of the above 6 kinds of compatibility proportion of Gualoupi-Gualouzi were 0.8808 ± 0.0407,0.8930 ± 0.0236,0.8995 ± 0.0195,0.9033 ± 0.0190,0.9363 ± 0.0082 and 0.8904 ± 0.0144,respectively. Among them,Gualoupi-Gualouzi ratio of 50：50 had the highest similarity,and compared with other groups,the differences were statistically significant（P〈0.05 or 0.01）. Conclusion The optimum proportion of Gualoupi-Gualouzi can be determined by the fingerprint technology,which provides the theoretical basis for the reasonable proportion of Gualou？pi-Gualouzi.

关 键 词：瓜蒌皮-瓜蒌子 配伍比例 指纹图谱 相似性 

分 类 号：R284[医药卫生—中药学]