转化生长因子β和IL-10对小鼠胸膜间皮细胞功能的影响  

Roles of TGF-β and IL-10 in murine pleural mesothelial cells

作　　者：王曜[1] 吴秀芝[1] 石欣雨[1] 王栋[1] 李婷[1] 王雯[1] 裴雪彬 姜帅[1] 施焕中[1] Wang Yao Wu Xiuzhi Shi Xinyu Wang Dong Li Ting Wang Wen Pei Xuebin Jiang Shuai Shi Huanzhong

机构地区：[1]首都医科大学附属北京朝阳医院呼吸与危重症科北京呼吸疾病研究所,100020

出　　处：《国际呼吸杂志》2017年第6期401-405,共5页International Journal of Respiration

基　　金：国家自然科学基金（91442109、31470883）

摘　　要：目的 探究转化生长因子β（TGF-β）和IL-10对胸膜间皮细胞（pleural mesothelial cells,PMCs）增殖、表面共刺激分子表达以及分泌一氧化氮（NO）功能的影响.方法 选择6～8周龄C57BL/6雌性野生型小鼠进行试验,通过胰酶消化法提取原代PMCs,加入TGF-β或IL-10刺激培养,运用流式细胞术检测PMCs增殖情况,细胞表面表达MHC-Ⅱ、CD86和CD80的变化以及检测培养上清中NO含量.结果 TGF-β可抑制PMCs增殖并下调MHC-Ⅱ和CD86的表达,对CD80表达及NO分泌无显著影响;IL-10对于PMCs的增殖,MHC-Ⅱ、CD80、CD86表达及分泌NO作用不明显.结论 TGF-β可抑制PMCs的增殖及抗原递呈功能,从而可能参与了胸腔积液的病理生理过程.Objective Transforming growth factor-β（TGF-β） and interleukin-10 （IL-10） were well-known anti-inflammatory cytokines and participate in pleural effusion immunity.The aim of our study was to investigate the roles of TGF-β and IL-10 in murine pleural mesothelial cells （PMCs）.Methods 6-8 weeks＇ old,female C57BL/6 wild type mice were used.PMCs were obtained by digestion with intrapleural injection of trypsin,then stimulated with TGF-β or IL-10 for 48 hours.The proliferation and the expression of MHC-Ⅱ,CD86 and CD80 on PMCs were detected and NO from the culture supernatant were measured.Results The purity of the PMCs were 99.76 %.TGF-β inhibited PMCs＇proliferation and weakened the expression of MHC-Ⅱ and CD86,while it had no significant effects on CD80 expression and NO secretion.There showed no significant difference when stimulated with IL-10 in all experiments.Conclusions TGF-β suppressed the proliferation and antigen presentation of PMCs,which might play an important role in the formation and development of pleural effusions.

关 键 词：炎症抑制因子 胸膜间皮细胞 胸腔积液 

分 类 号：R561[医药卫生—呼吸系统]