鲍曼不动杆菌生物被膜形成与相关基因的研究  

Correlations of Acinetobacter baumannii biofilms to related genes

作　　者：冯雁京 刘原[1] 柯蕊[1] 和平[1] 张立然[1] 杨妮[2] 敬蓉[1] 马淑珍[1] 刘聪蕊 Feng Yanjing Liu Yuan Ke Rui He Ping Zhang Liran Yang Ni Jing Rong Ma Shuzhen Liu Congrui(Department of Respiratory Medicine, the Second Affiliated Hospital of Medical College, Xi＇an J iaotong University, Xi＇an 710004, China)

机构地区：[1]西安交通大学第二附属医院呼吸内科,710004 [2]西安交通大学第二附属医院急诊科,710004

出　　处：《国际呼吸杂志》2017年第6期428-431,共4页International Journal of Respiration

基　　金：国家自然科学基金（81270061）;陕西省科技攻关项目[2010K16-01（02）]

摘　　要：目的 分析鲍曼不动杆菌生物被膜形成能力与生物被膜相关基因之间的关系.方法 采用结晶紫染色法检测菌株生物被膜形成能力,并应用聚合酶链反应（PCR）方法检测菌株生物被膜相关基因的表达情况.结果 66.13％的鲍曼不动杆菌可形成生物被膜.生物被膜形成相关基因csuC、csuD、csuE、bap、bfms及aba I扩增阳性率分别为87.9％、91.1％、79.0％、50.8％、70.2％、60.5％.除csuC、abaI基因外,生物被膜形成能力阳性株csuD、csuE、bap、bfms基因阳性率高于生物被膜形成能力阴性株（P＜0.05）.结论 鲍曼不动杆菌生物被膜形成能力与csuD、csuE、bap、bfms基因密切相关.Objective To study the correlation between A.baumannii.Biofilm formation ability and biofilm related genes.Methods The crystal-violet straning method was applied to estimate the biofilm formation ability.Polymerse chain reaction （PCR） was used to test the expression of biofilm formation associated genes.Results Majority of A.baumannii.strains formed biofilm in vitro.87.9%,91.1%,79.0%,50.8%,70.2%,60.5% of strains expressed csuC,csuD,csuE,bap,bfms and aba I genes respectively.Except for csuC and abaI gene,csuD、csuE、bap、bfms genes expression rates were higher in biofilm-forming strains than their non-biofilm-forming counterparts （P 〈 0.05）.Conclusions Biofilm formation ability of A.baumannii is closely correlated with csuD,csuE,bap and bfms genes.

关 键 词：鲍曼不动杆菌 生物被膜 生物被膜相关基因 

分 类 号：R446.5[医药卫生—诊断学]