藏药绿萝花水溶性多糖提取工艺的研究  

Study on the extraction technology of water-soluble polysaccharide from Tibetan medicine Scindapsus aureus(Linden ex Andre)

作　　者：孙翠翠[1] 程江[2] 唐棣[2] 刘群[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]四川省兽药监察所,成都610041

出　　处：《黑龙江畜牧兽医》2017年第4期208-213,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2011BAD34B02-4);西南民族大学研究生创新型科研项目(CX2015SZ090)

摘　　要：为了优化绿萝花水溶性多糖的提取工艺,试验以不同料液比、提取温度、提取时间为自变量,以绿萝花水溶性多糖提取率为响应值,在单因素试验基础上,运用中心组合设计(Box-Behnken)三因素三水平的响应面分析方法,研究各自变量及交互项对绿萝花水溶性多糖提取率的影响。结果表明:绿萝花多糖最佳提取条件为料液比1∶20、温度50℃、时间4 h,在此优化条件下绿萝花多糖最佳提取率为8.82%。To optimize the extraction technology of water - soluble polysaccharides from Scindapsus auretts （ Linden ex Andre）, different solid - liquid ratios, extraction temperature, extraction time were used as the independent variables, and the extract rate of water - soluble polysaccha- ride from Scindapsus aureus （ Linden ex Andre） as response value. A method of three - factor and three - level response surface analysis with central composite design （ Box - Behnken） was used to study the effects of various variables and their interaction on the extract rate of water - soluble polysaccharide from Scindapsus aureus （ Linden ex Andre） based on the single factor experiment. The results showed that the optimum extraction conditions of polysaccharide from Scinclapsus aureus （ Linden ex Andre） were as follows ： solid - liquid ratio of 1：20, extraction tem- perature of 50 ℃, extraction time of 4 h. The optimal extraction rate of polysaccharide from Scindapsus aureus （Linden ex Andre） was 8.82% under the optimized conditions.

关 键 词：绿萝花水溶性多糖 单因素试验 中心组合设计 响应面分析 提取率 

分 类 号：R284.2[医药卫生—中药学]