二氢杨梅素及其氧钒配合物与DNA的相互作用研究  

DNA-binding of Dihydromyricetin and Its Oxovanadium(Ⅳ) Complex

作　　者：严高剑[1,2] 叶振锋[2] 柴栋[1] 唐敬[2] 陆家政[2] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东药科大学药学院,广东广州510006

出　　处：《化学试剂》2017年第4期409-412,420,共5页Chemical Reagents

基　　金：广东省科技计划项目(2012B031800431);广东药大学自然科学联合基金资助项目(2014-36)

摘　　要：二氢杨梅素是藤茶中重要的药效物质。合成了一种二氢杨梅素的氧钒配合物,其结构经元素分析、IR、ESI-MS、~1HNMR和^(13)CNMR表征。采用紫外分光光度法、荧光淬灭和流体力学等方法分别测定二氢杨梅素及其氧钒配合物与DNA的键合能力。结果显示,这两种化合物都能以插入方式与CT-DNA结合,DNA断裂实验证实该新型配合物可较好地断裂p BR322 DNA。Dihydromyricetin is an important pharmacodynamic material in Tengcha.Its oxovanadium complex（VO-DMY） has been synthesized and confirmed by elemental analysis,IR,UV-Vis,ESI-MS,^1HNMR and ^（13）CNMR.The DNA-binding activity of DMY and VO-DMY with calf thymus CT-DNA have been investigated by using UV-Vis absorption titration,fluorescence spectra and viscosity measurement.The results indicate that DMY and VO-DMY can bind to CT-DNA via an intercalative mode and can efficiently cleave pBR322 DNA after DNA photo cleavage experiment.

关 键 词：二氢杨梅素 氧钒配合物 DNA键合 

分 类 号：R284[医药卫生—中药学]