锰对SH-SY5Y细胞线粒体超微结构及功能改变的研究  

Mitochondrial ultrastructure and function changes in SH-SY5Y induced by manganese

作　　者：宋冬梅[1] 陈丽[1] 马俊香[1] 张媛媛[1] 张诗玄 郭彩霞[1] 陈田[1] 田琳[1] 牛丕业[1] 

机构地区：[1]首都医科大学公共卫生学院环境毒理学北京市重点实验室,北京市100069

出　　处：《实用医学杂志》2017年第5期680-683,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81673137);北京市自然科学基金项目(编号:7172024);北京市教育委员会高等学校高层次人才引进与培养及创新团队建设计划项目(编号:CIT&TCD201504094)

摘　　要：目的:探讨锰染毒对SH-SY5Y细胞线粒体超微结构的影响及线粒体功能的改变。方法:透射电镜观察对照组和MnCl_2处理组(250μmol/L,6 h)SH-SY5Y细胞线粒体形态的变化,流式细胞术检测细胞膜电位变化,以及荧光酶标仪检测细胞内活性氧的含量。结果:与对照组相比,MnCl_2处理组线粒体形态发生明显的肿胀、空泡化、基质浓缩明显,线粒体嵴数减少或消失;细胞线粒体膜电位在250μmol/L作用6 h后明显降低(P<0.05);同时在相同处理条件下,ROS产生水平较对照组有明显升高(P<0.05)。结论:锰可以损伤线粒体的超微结构并影响其功能。Objective To explore the mechanism of SH- SY5Y mitochondrial dysfunction treated by manganese to find a new potential therapeutic target. Methods Transmission Electron Microscopy（TEM）to observe the morphology of mitochondria. Cell treated with 250 μmol/L for periods of time （2 h, 4 h, 6 h） while mitochondrial membrane potential （MMP） and ROS can be detected by FCM and fluorescence microplate reader. Results After treating with MnCl2 in 6 h, TEM images showed early vacuoles, lamellar structures of SH-SY5Y cells. Then test the mitochondrial membrane potential and showed that MMP would be decreased gradually. Meanwhile, analysis showed that in comparison with control, treatment group had a higher ROS level respectively （P 〈 0.05）. Conclusion MnC12 can cause mitochondrial damage through a mechanism closely related to disrupt the MMP or generate abundant ROS.

关 键 词：锰 线粒体 超微结构 线粒体膜电位 

分 类 号：R114[医药卫生—卫生毒理学]