厦门、深圳及广州鼠形动物脑组织标本基孔肯雅病毒携带情况调查  

A survey of chikungunya virus in brain tissue samples from rat like animals in Xiamen, Shenzhen and Guangzhou

作　　者：霍舒婷 莫云[1] 师永霞[2] 熊益权[1] 李东亮[1] 邱旻[1] 郑雪燕[1] 陈少威[1] 蒋丽娜[1] 陈清[1] 

机构地区：[1]南方医科大学公共卫生学院流行病学系,广州市510515 [2]广东出入境检验检疫局检验检疫技术中心,广州市510623

出　　处：《实用医学杂志》2017年第5期820-822,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81373051)

摘　　要：目的:了解厦门、深圳及广州的鼠形动物脑组织标本基孔肯雅病毒(CHIKV)的携带情况,分析鼠形动物作为人类CHIKV宿主和潜在传染源的可能性。方法:选取2013年1月至2016年6月在厦门、深圳及广州(白云区、越秀区)部分居民区、城市公园、医院、农贸市场、学校捕获鼠形动物,无菌采集其脑组织样本,用逆转录聚合酶链反应(RT-PCR)方法检测CHIKV。结果:本次调查共捕获鼠形动物1 092只2目2科3属7种,家鼠以褐家鼠为优势鼠种,野鼠以黄毛鼠为优势鼠种,共获1 092份脑组织标本,RT-PCR方法未在采集的脑组织标本中检测出CHIKV。结论:厦门、深圳及广州鼠形动物作为CHIKV宿主和传染源的可能性小。Objective To examine the prevalence of chikungunya virus in brain tissue samples from rat- like animals in Xiamen, Shenzhen and Guangzhou, and to explore whether the rat-like animals are potential sources of human chikungunya fever infections and the host of the virus. Methods Rat-like animals were trapped in residential areas, city parks, hospitals, markets and schools in Xiamen, Shenzhen and Guangzhou （Yuexiu and Baiyun districts） between January 2013 and June 2016. Brain tissue samples of the trapped animals were collected under sterile. Chikungunya virus was detected by using reverse transcription polymerase chain reaction （RT-PCR）. Results Totally 1092 rat-like animals were trapped, which belonged to 7 species, 3 genera, 2 families, 2 orders. Rattus norvegicus was the dominant species in the indoor environment, Rattus losea was dominant in wild environment, and 1092 brain tissue samples were collected. No detectable chikungunya virus was found in the brain tissue samples by RT-PCR. Conclusion There is a low possibility that rat-like animals act infectious sources of human chikungunya fever infections and the host of the virus.

关 键 词：基孔肯雅病毒 鼠形动物 脑组织 

分 类 号：R512.8[医药卫生—内科学] R181.3[医药卫生—临床医学]