乌司他丁预处理可减轻氧糖剥夺诱导的GES-1细胞损伤  

Preconditioning of ulinastatin alleviates GES-1 cell injury induced by oxygen and glucose deprivation

作　　者：王瑶[1] 郄文斌[1] 吴友平[1] 贾济[1] 屠伟峰[1] 

机构地区：[1]南方医科大学附属广州军区广州总医院麻醉科,广州市510010

出　　处：《实用医学杂志》2017年第6期858-862,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:81272141);广东省自然科学基金重点项目(编号:2014A030311012)

摘　　要：目的:观察乌司他丁(UTI)预处理对氧糖剥夺(OGD)诱导人胃黏膜上皮细胞株(GES-1)细胞损伤的影响。方法:采用GES-1细胞株进行实验,设置正常对照组(N组)、氧糖剥夺组(O组)、乌司他丁预处理组(U组)。N组不做任何处理,O组和U组通过三气培养箱和无糖培养基共同作用造成细胞氧糖剥夺损伤,U组在损伤前12 h预先加入乌司他丁处理。损伤处理6 h后,采用CCK-8法测定细胞活力,流式细胞术检测细胞凋亡率,免疫印迹法检测Caspase-3和Cleaved Caspase-3的蛋白表达水平,实时荧光定量PCR法检测细胞内瞬时受体电位香草酸亚型-1(TRPV1)分子m RNA的表达水平。结果:与N组比较,O组细胞存活率显著降低,细胞凋亡率明显升高,Caspase-3和Cleaved Caspase-3表达增加,TRPV1 m RNA表达降低(P<0.05);乌司他丁预处理能显著抑制上述O组的变化,差异有统计学意义。结论:乌司他丁预处理可减轻OGD诱导的GES-1细胞损伤,其机制可能与抑制细胞凋亡以及调控TRPV1的表达有关。Objective To observe the effects of the preconditioning of ulinastatin on GES- 1 cell injury induced by oxygen and glucose deprivation （OGD）. Methods GES-1 cells were cultured in vitro and divided into three groups： normal control group （group N）, oxygen and glucose deprivation group （group 0）, and ulinastatin preconditioning group （group U）. The OGD model of GES-1 ceils were established by glucose-free medium and three -gas incubator for 6h. Ulinastatin was added to group U 12h before the deprivation of oxygen and glucose. The cell viability and apoptosis were determined by cck- 8 and flow cytometry respectively. Western Blot was used to examine the protein expression of Caspase-3 and Cleaved Caspase-3. The TRPV1 mRNA expression was measured by quantitative real-time PCR. Results As compared with group N, the viability of GES- 1 was decreased, the apoptotic rate and the expression of Caspase-3 and Cleaved Caspase-3 were increased, and the TRPV1 mRNA expression decreased greatly in group O （P 〈 0.05）. As compared with group O, the aforementioned changes were significantly inhibited in group U. Conclusions Ulinastatin preconditioning could effectively inhibit GES-1 cell injury induced by OGD, which may be related to the inhibition of apoptosis and the upregulation of TRPV 1 mRNA expression.

关 键 词：乌司他丁 预处理 GES-1细胞 氧糖剥夺 

分 类 号：R61[医药卫生—外科学]