精杞胶囊对大鼠生精功能损伤的保护作用  

Protective effects of Jingqi Capsules on damage to rat reproductive system

作　　者：万芳[1] 马红[1] 吕晔[2] 韦敏[2] 

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《中成药》2017年第4期672-676,共5页Chinese Traditional Patent Medicine

基　　金：江苏省高校优势学科建设工程一期项目(YS2012ZYX304)

摘　　要：目的探讨精杞胶囊(蛹虫草、熟地、枸杞子,等)对环磷酰胺诱导大鼠生精功能损伤的保护作用。方法精杞组和丙酸睾酮组先期给药2周后,除正常组外,均腹腔注射环磷酰胺25 mg/(kg·d)连续造模5 d,与此同时精杞组和丙酸睾酮组延续给药,造模结束后次日处死全部动物。放射性免疫法检测血清睾酮含有量;称取大鼠体质量、睾丸、附睾、前列腺质量,计算脏器指数;HE染色观察大鼠睾丸组织形态;CASA仪检测精子密度与精子活率;TUNEL检测细胞凋亡。结果精杞组与模型组比较,血清睾酮含有量升高;睾丸、附睾、前列腺脏器指数增加;生精上皮损伤减轻,各级生精细胞数量增多;精子密度与精子活率增高;TUNEL显示细胞凋亡率降低。结论精杞胶囊对环磷酰胺所致大鼠生精功能损伤有保护作用。AIM To study the protective effects of Jingqi Capsules （Cordycps Militaris, Rehmanniae Radix Praeparata, Lycii Fructus, ） on the damage to rat reproductive system induced by cyclophosphamide. METHODSAfter low and high dose groups of Jingqi Capsules and positive group were administered for two weeks in ad-vance, cyclophosphamide [ 25 mg/（ kg · d） ] modeling was conducted consecutively by intraperitoneal injection for five days except the normal group, while the treatment groups were administered consecutively. All the rats were executed the day after the end of the modeling. Serum testosterone content was measured by radioimmunoassay; body weight, testis, epididymis and prostate of rats were weighed and organ coefficient was calculated ; morpholo-gy of testis was observed by HE; sperm count and sperm motility were detected by CASA; apoptosis of cells was observed by in the level TUNEL. RESULTS Compared with the model group, each dose group of Jingqi Capsules increased of serum testosterone and organ coefficient of testis epididymis and prostate. The damage to seminiferous epithelium relieved and the number of all spermatogenic cells increased. Sperm count and sperm motility increased. Decrease in apoptosis of cells was demonstrated by TUNEL. CONCLUSION Jingqi Capsules has protective effects on damage to rat reproductive system induced by cyclophosphamide.

关 键 词：精杞胶囊 环磷酰胺 生殖损伤保护 生精功能 

分 类 号：R285.5[医药卫生—中药学]