坤泰胶囊对VCD所致POI模型大鼠的疗效探究  

Curative effect of Kuntai Capsules on POI rats induced by VCD

作　　者：周蓓蓓[1] 陈文俊[2] 谈勇[1] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]浙江省中医院,浙江杭州310006

出　　处：《中成药》2017年第4期695-700,共6页Chinese Traditional Patent Medicine

基　　金：江苏省科技计划项目临床医学科技专项(BL2013039);横向课题;和颜坤泰胶囊对VCD所致大鼠卵巢功能损伤动物模型的保护作用研究(2015026)

摘　　要：目的探究坤泰胶囊(黄连、黄芩、阿胶,等)对去氧乙烯基环乙烯(VCD)所致卵巢功能不全(POI)模型大鼠的疗效。方法 75只21日龄离乳大鼠,随机均分为佐剂对照组,模型组,坤泰胶囊低、中、高剂量组。除佐剂对照组腹腔注射芝麻油外,其余4组均给予腹腔注射VCD溶液。灌胃给药45 d。每组选取8只持续处于动情间期或持续动情周期紊乱的大鼠,进行检测大鼠血清促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E_2)、抗苗勒管激素(AMH);剖腹取出卵巢、子宫称重并进行形态学观察。结果与佐剂对照组相比,模型组大鼠血清FSH明显增高且AMH、E_2显著降低,LH有所增高;经坤泰中、高剂量干预后,FSH水平显著降低,AMH水平明显增加;模型组大鼠卵巢湿重较佐剂对照组减轻,坤泰胶囊干预后,低剂量组卵巢湿重增加明显;模型组大鼠各级卵泡及黄体计数均明显减少,坤泰胶囊干预后,各级卵泡计数明显增加,黄体计数有所增加;光镜下,模型组子宫内膜变薄,管壁子宫腺体数量明显减少。经坤泰胶囊干预后,内膜有所增厚,腺体数量明显增加。结论坤泰胶囊可以减缓模型大鼠卵巢功能衰减的速度。AIM To investigate the curative effects of Kuntai Capsules （ Coptidis Rhizoma, Scutellariae Radix, Asini Corii Colla, etc. ） on rat model for primary ovarian insufficiency （POI） caused by 4-vinylcyclohexene diepoxide （VCD）. METHODS Seventy-five weaned 21-day old rats were divided equally into five groups randomly： control group, model group, low-, medium-, and high-dosage Kuntain group. Except the control group was given sesame oil rats, other four groups were given intraperitoneal injection of VCD solution. According to vaginal smear of rats, eight rats in each group were selected, the levels of FSH （follicle-stimulating hormone）, LH （leuteinizing hormone）, E2 （estradiol） and AMH（ anti-Mullerian hormone） were measured, and morphological changes of ovary and uterus were observed. RESULTS Compared to the control group, FSH level in rats＇ serum of the model group significantly increased while AMH and E2 levels significantly decreased, and LH level rose; Kuntai Capsules significantly decreased FSH level while increased AMH level ; The wet weight of rats＇ ovary in the model group was lighter than that in the control group. The ovary weight of Kuntai Capsules low-dosage group was increased ; the number of follicles at all levels and corpora lutea of rats in the model group were decreased. After the treatment with Kuntai Capsules, the number of follicles at all levels and corpora lutea were increased ; light microscopy manifested decreased uterine glands of the tube wall, reduced glandular cavity. After the treatment with Kuntai Capsules, the number of uterine glands increased and the gland cavity expanded. CONCLUSION Kuntai Capsules may slowdown the functional decline rate of ovarian.

关 键 词：坤泰胶囊 卵巢功能不全(POI) 去氧乙烯基环乙烯(VCD) 

分 类 号：R285.5[医药卫生—中药学]