HPLC法同时测定桑芪首乌片中7种成分  

Simultaneous determination of seven constituents in Sangqishouwu Tablets by HPLC

作　　者：李争艳[1] 袁小淋 魏丹霞[1] 李月亭[2] 余晓玲[1] 

机构地区：[1]昆明市中医医院,云南昆明650051 [2]云南中医学院,云南昆明650200

出　　处：《中成药》2017年第4期737-740,共4页Chinese Traditional Patent Medicine

基　　金：云南省计划联盟项目(2012CG031)

摘　　要：目的建立HPLC法同时测定桑芪首乌片(桑寄生、黄芪、制何首乌等)中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、氧化芍药苷、芍药内酯苷和苯甲酰芍药苷的含有量。方法该药物甲醇提取液的分析采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.02%磷酸,梯度洗脱;体积流量0.9 mL/min,检测波长230、254 nm;柱温25℃。结果 7种成分在各自范围内均呈良好的线性关系(r≥0.999 2),平均加样回收率97.13%~100.03%,RSD 0.69%~1.47%。结论该方法快速、灵敏、准确、专属性好,可用于桑芪首乌片的质量控制。AIM To establish an HPLC method for the simultaneous content determination of calycosin-7-glucoside, ononin, calycosin, formononetin, oxypaeoniflorin, alibiflorin and benzoylpaeoniflorin in Sangqishouwu Tablets （ Talxilli Herba, Astragali Radix, Polygoni multiflori Radix Praeparata, etc. ）. METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Diamonsil Cls eolmn （250 mm × 4. 6 mm, 5 μm）, with the mobile phase comprising of acetonitrile- 0. 02% phosphoric acid flowing at 0. 9 mL/min in a gradient elution manner, and the detection wavelengths were set at 230 nm and 254 nm. RESULTS Seven constituents showed good linear relationships within their own ranges （ r ≥ 0. 999 2 ） , whose average recoveries were 97.13% - 100. 03% with the RSDs of 0. 69% - 1.47%. CONCLUSION This sensitive, accurate and specific method can be used for the rapid quality control of Sangqishouwu Tablets.

关 键 词：桑芪首乌片 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]