HPLC法同时测定5个采收期蛇莓中5种黄酮成分  

Simultaneous determination of five flavonoids in Duchesnea indica at five picking time by HPLC

作　　者：王治阳 张峰[1] 代震[1] 安华[1] 

机构地区：[1]河南省中医药研究院附属医院,河南郑州450004

出　　处：《中成药》2017年第4期786-789,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定5个采收期(4、5、6、7、8月)蛇莓Duchesnea indica(Andr.)Focke中5种黄酮成分的含有量。方法蛇莓甲醇提取液的分析采用Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%甲酸为流动相,梯度洗脱;体积流量1.0 mL/min;检测波长350 nm;柱温25℃。结果芦丁、金丝桃苷、异槲皮素、洋芹素和山柰酚在各自范围内均呈良好的线性关系(R^2≥0.998 6),平均加样回收率97.1%~101.5%,RSD 1.37%~2.37%。不同采收期样品中5种成分含有量差异明显,其中芦丁和金丝桃苷在6月份最高,异槲皮素在7月份最高,洋芹素和山柰酚在8月份最高。结论蛇莓适宜采收期为6月和7月。AIM To establish an HPLC method for the simultaneous content determination of five flavonoids in Duchesnea indica （Andr.） Focke at five picking time （April, May, June, July and August）. METHODS The analysis of D. indica methanol extract was performed on a 25 ℃ thermostatic Agilent ZORBAX SB-C18 column （250 mm × 4.6 mm, 5 μm）, the mobile phase comprising of acetointrile.-0. 1% methanoic acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 350 nm. RESULTS Rutin, hyperoside, isoquercitrin, celereoin and kaempferol showed good linear relationships within their own ranges （R2 ≥0. 998 6）, whose average recoveries were 97.1% - 101.5% with the RSDs of 1.37% -2.37% . The contents of five constituents in samples at different picking time exhibited obvious differences, among which lutin and hyperoside contents were the highest in June, isoquercitrin content was the highest in July, and eelereoin and kaempferel contents were the highest in August. CONCLUSION The suitable niekin, time of D. indica is June and July.

关 键 词：蛇莓 采收期 芦丁 金丝桃苷 异槲皮素 洋芹素 山柰酚 HPLC 

分 类 号：R284.1[医药卫生—中药学]