实时荧光PCR法和流式细胞术检测HLA-B27的结果分析  

Analysis on HLA B27 results detected by real-time fluorescence PCR method and flow cytometry

作　　者：叶阿里[1] 窦亚玲[1] 孔令君[1] 张睿[1] 伊洁[1] 

机构地区：[1]中国医学科学院北京协和医院检验科,北京100730

出　　处：《国际检验医学杂志》2017年第7期892-894,共3页International Journal of Laboratory Medicine

摘　　要：目的比较实时荧光PCR法和流式细胞术两种方法检测人类白细胞抗原B27(HLA-B27)的临床应用价值。方法分别采用实时荧光PCR法和流式细胞术两种方法检测225例疑似强直性脊柱炎患者血中HLA-B27,比较和分析两种方法的检测结果。结果 95.11%样本检测结果相同,差异不具有统计学意义(P>0.05),将存在差异的标本进行基因测序后,结果与实时荧光PCR的检测结果一致。结论两种方法检测HLA-B27均具有较高的灵敏度和特异度,实时荧光PCR法在结果的准确性上更优于流式细胞术。Objective To compare the clinical application value of the real-time fluorescence PCR method and flow cytometry method for detecting human leukocyte antigen B27（HLA-B27）. Methods Blood HLA-B27 level in 225 patients with suspected an- kylosing spondylitis was detected by using real-time fluorescence PCR method and flow cytometry method. The detection results were compared and analyzed between the two methods. Results The results of 95.1 % sample were identical detected by two methods without statistically significant difference（P〉0.05）. Taken the results of flow cytometry as reference, the sensitivity of realtime fluorescence PCR for detecting HLA-B27 was 94%, the specificity was 96%. Gene sequencing was performed if results of a sample detected by two methods were different, which was identical with the result detected by real-time fluorescence PCR. Conclusion Both methods for detecting HLA- B27 all have high sensitivity and specificity. Real-time fluorescence PCR method is more superior to the flow cytometry method in the results accuracy.

关 键 词：HLA-B27 荧光PCR 流式细胞术 脊柱炎 

分 类 号：R440[医药卫生—诊断学] R446[医药卫生—临床医学]