BBD-响应面法优化黑果枸杞色素的渗漉法提取工艺及其纯化工艺研究  

The BBD-response surface method process of percolation extraction process optimization and purification of Lycium ruthenicum pigment

作　　者：娄涛涛 金玲[1] 陀扬凌 陈天强 张旭[1] 

机构地区：[1]成都中医药大学药学院教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都610075 [2]青海天强中医药科技开发有限公司,格尔木816000

出　　处：《食品科技》2017年第3期244-250,共7页Food Science and Technology

基　　金：成都市科学技术局项目(2015-HM01-00188-SF)

摘　　要：目的:采用BBD-响应面法优化黑果枸杞色素的渗漉法提取工艺,并对其纯化工艺进行研究。方法:采用单因素实验结合BBD-响应面法,对影响渗漉法提取的3个主要因素乙醇浓度、渗漉速度、溶剂倍数进行了优化。通过筛选出吸附性能最好的大孔树脂,对影响其吸附与解析的因素:上样液浓度、上样液pH值、径高比、上样流速、水洗用量、洗脱剂浓度、洗脱剂pH值、洗脱剂流速进行考察,并对色素色价和产率进行测定。结果:最佳工艺是采用pH3.0,20倍量79%乙醇,以1 mL/min的流速进行渗漉提取。采用X-5大孔树脂纯化色素,径高比1:15、色素液浓度0.02 g/mL,上样液pH3.0、上样量90 mL,流速3 mL/min为最佳吸附条件;以5 BV的95%乙醇在pH2.0、流速3 mL/min的条件下洗脱效果最佳,纯化色素产率可达7.33%,色价为21.7,重复性较好,适合于工业化生产。结论:该工艺操作简单、重复性好,节约成本,可为色素大工业生产提供参考。Objective： To investigate the application BBD-response surface methodology to optimize the pigment of Lycium ruthenicum Murr infiltration percolation method extraction process, and the purification process was studied. Method： By single factor experiment combined with BBD-response surface method, the influence on infiltration percolation method to extract the three main factors of ethanol volume fraction, infiltration percolation velocity and solvent fold amount were optimized. Through the screening of the best macroporous resin adsorption properties, the adsorption and analytical results were screened and purified. Factors of effect of macroporous resin adsorption and desorption concentration of sample solution, sample solution pH, ratio of diameter to height, sample flow rate, amount of washing, elution solvent concentration, elution solvent pH value, elution solvent flow rate were investigated, and the color value and yield were determined. Results： The optimum process was pH 3.0, 20 times the amount of 79% ethanol at flow rate of 1 mL/min for percolation extraction. With X-5 macroporous resin, diameter to height ratio 1：15, pigment concentration of 0.02 g/mL, sample solution pH 3.0, the quantity of sample was 90 mL, flow rate of 3 mL/min for the optimum adsorption conditions; under the condition of 5BV of 95% ethanol in pH 2.0, flow rate of 3 mL/min elution effect was the best. The purified pigment yield could reach 7.33%, color value was 21.7, good repeatability, and was suitable for industrialized production. Conclusion： The process was simple, reproducible, and cost saving, which could provide reference for large industrial production.

关 键 词：黑果枸杞 色素 渗漉提取 BBD-响应面法 纯化 

分 类 号：R284[医药卫生—中药学] TS202.3[医药卫生—中医学]