荧光编码微球的制备研究进展  

Progress in the Preparation of Fluorescence-Encoded Microspheres

作　　者：李艳[1] 罗成[1] 吴道澄[2] 

机构地区：[1]宜春学院医学院,江西宜春336000 [2]西安交通大学生命科学与技术学院教育部生物医学信息工程重点实验室,西安710049

出　　处：《中国生物医学工程学报》2017年第2期219-227,共9页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(81471771);江西省自然科学基金(20161BAB215196)

摘　　要：悬浮芯片技术是近年来兴起的高通量生物检测技术,在生命科学研究及临床诊断等领域具有广泛应用。作为悬浮芯片技术的检测载体,荧光编码微球的研究已取得一系列进展。首先对悬浮芯片技术的检测原理及应用进行简单介绍,重点对荧光编码微球的制备方法进行总结,包括有机溶剂溶胀法、层-层自组装法、包埋法、微流控技术、膜乳化法等,最后对荧光编码微球未来的发展趋势进行探讨。Suspension array is a promising platform for multiplex analyses with broad applications in science research and clinical diagnosis. As one of the key components for the suspension array, fluorescence-encoded microspheres have received much attention and a lot of achievements have been made. In this review, the analysis pattern and applications of suspension array were introduced, and recent advances in the fluorescence- encoded microspheres were summarized according to the classification of preparation methods including swelling method, layer-by-layer （LBL） self-assembly, embedding in microspheres, microfluidic techniques and membrane emulsification. Current challenges and future directions of fluorescence-encoded microspheres were also outlined.

关 键 词：悬浮芯片 微球 荧光编码 量子点 

分 类 号：R318[医药卫生—生物医学工程]