甘蔗内标基因PCR检测技术研究  

PCR Detection Technology of Endogenous Reference Genes of Sugarcane

作　　者：肖乃衍 林冰[1] 刘迪[1] 陈平华[1,2] 许跃语 施桂姣[1] 王恒波[1,2] 郭晋隆[3,2] 高三基[1] 许莉萍[3] 陈如凯[1] 

机构地区：[1]福建农林大学国家甘蔗工程技术研究中心,福建福州350002 [2]农业部甘蔗及制品质量监督检验测试中心转基因检测室,福建福州350002 [3]农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350002

出　　处：《热带作物学报》2017年第3期500-507,共8页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金(No.2014J01092);国家自然科学基金项目(No.31070330);科技发展基金项目(No.KF2015080;KF2015118)

摘　　要：克隆鉴定甘蔗内源低拷贝基因鲜有研究,影响转基因甘蔗精确鉴定。根据其他作物已有的内标基因和内源低拷贝基因,按照内标基因的物种特异性和通用性原则,用12个有代表性甘蔗栽培品种对11个候选基因进行筛选与鉴定,获得了甘蔗特有的内源低拷贝基因,并以该基因建立了甘蔗内标基因PCR检测技术体系,为转基因甘蔗的精确检测和转基因标准的制定奠定了基础。Cloning and identification of endogenous low copy gene in sugarcane has been rarely reported, which affects the accurate determination of the transgenic sugarcane. Low copy endogenous genes of sugarcane were identified referring to the known endogenous reference genes and low copy endogenous genes of other crops. Eleven candidate genes were tested with twelve representative sugarcane cultivars based on the principles of species specific and universal existence. PCR protocol to amplify the low copy endogenous gene was set up subsequently, which contributes to identify transgenic sugarcane plants precisely and lays the foundation for development of relevant detection standards.

关 键 词：甘蔗 内标基因 鉴定 PCR检测技术 

分 类 号：S566.103[农业科学—作物学]