补肾中药单体对氧化损伤内皮细胞中p66mRNA的调控作用  

R egulating Effect of Tonifying Kidney Monomer on p66mRNA of Oxidative Endothelial Cells

作　　者：孙申维 顾闻[1] 陈川[2] 刘特[2] 陈久林[2] 申定珠[2] 邢三丽[2] 郁志华[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海市中医老年医学研究所,上海200031

出　　处：《四川中医》2017年第3期40-43,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81373706)

摘　　要：目的:研究京尼平苷酸、齐墩果酸、补骨脂素、芝麻素、特女贞苷、肉桂酸6种补肾中药单体对氧化损伤内皮细胞中p66mRNA的调控,以探讨其抗氧化、保护血管内皮细胞的机制。方法:采用H_2O_2诱导EA.hy926(人脐静脉细胞融合细胞)为血管内皮细胞氧化应激损伤模型,予不同浓度的京尼平苷酸、齐墩果酸、补骨脂素、芝麻素、特女贞苷、肉桂酸干预后,用qPCR法检测不同单体对氧化损伤内皮细胞中p66mRNA的作用。结果:H_2O_2刺激EA.hy926后引起细胞内SOD活性降低,MDA含量增高,活性氧含量增高。5μM、50μM、500μM的京尼平苷酸、齐墩果酸、补骨脂素、芝麻素、特女贞苷、肉桂酸均能抑制血管内皮细胞氧化应激损伤模型中p66mRNA表达水平,其中以特女贞苷、齐墩果酸的作用效果最为明显。结论:补肾中药单体抗氧化、保护血管内皮细胞的作用与调节p66mRNA有关。Objective： To study the regulation of p66 mRNA of six kinds of tonifying kidney monomer： geniposidic acid, oleanolic acid, psoralen, sesamin, specnuezhenide, cinnamic acid on oxidative endothelial cells and to explore the mechanisms of its anti-oxidation and protection of vascular endothelial cells. Methods： EA. hy926 （human umbilical vein endothelial cells） were induced by H2O2 to be the oxidative stress damage model of vascular endothelial cells. After the intervention of different con- centrations of geniposidic acid, oleanolic acid, psoralen, sesamin, specnuezhenide, cinnamic acid, p66 mRNA in the oxidative endothelial cells was detected by qPCR assay. Results： The superoxide dismutase （SOD） activity was decreased. The content of malondialdehyde （MDA） and reactive oxygen species was increased after the stimulation of H2O2. 5μM, 50μM, 500μM of geni- posidic acid, psoralen, sesamin, cinnamic, especially acid specnuezhenide and oleanolic acid, inhibited the level of 1366 mRNA expression level of oxidative vascular endothelial cells model. Conclusions ： The effect of anti-oxidation and protection of vascular endothelial cells of tonifying kidney monomer is related with the regulation of p66 mRNA expression level.

关 键 词：补肾中药单体 EA.hy926 氧化应激 P66SHC 

分 类 号：R285.5[医药卫生—中药学]