脂筏在牛呼吸道合胞体病毒感染MDBK细胞中的作用  

Lipid rafts play an important role in the infection of bovine respiratory syncytial virus

作　　者：李丽阳[1,2] 刘洋[1] 王磊[1] 李艳婷[2] 侯喜林[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2017年第4期643-647,共5页Chinese Journal of Veterinary Science

基　　金：黑龙江八一农垦大学校内培育课题资助项目(XZR2014-14);黑龙江省教育厅课题资助项目(12521372);国家科技计划课题资助项目(2012BAD12B05-2)

摘　　要：为探讨脂筏在牛呼吸道合胞体病毒(bovine respiratory syncytial virus,BRSV)感染MDBK细胞中的作用,采用胆固醇去除药物MβCD处理MDBK细胞。结果显示,细胞膜胆固醇含量明显降低,同时也降低BRSV感染水平,补充外源胆固醇后,病毒感染力可部分恢复;表明细胞膜胆固醇的去除可以抑制BRSV的感染,BRSV的感染与细胞膜脂筏相关。进一步研究发现,胆固醇去除不会影响BRSV结合在靶细胞表面,但会抑制病毒进入靶细胞。在病毒感染后采用胆固醇去除药物MβCD处理MDBK细胞,对于病毒感染力的影响是轻微的。这些发现可有助于理解BRSV的感染机制,为研制安全、高效的疫苗和有效的治疗药物提供理论依据。Bovine respiratory syncytial virus（BRSV） represents a significantly economical challenge to the cattle industry worldwide. In this study, we investigated the importance of cellular lipid rafts in BRSV infection. First,BRSV infection was decreased when cell membrance cholesterol was dele-ted by MβCD, demonstrating that infection of BRSV was associated with lipid rafts during viral entry,however after cholesterol replenishment, virus entry and subsequent replication rates were similar to the control infection. Furthermore cholesterol depletion did not significantly influence vi- rus binding and the post-entry replication stage. These findings might provide an insight into our understanding of of BRSV infection mechanism.

关 键 词：牛呼吸道合胞体病毒 脂筏 MβCD MDBK细胞 

分 类 号：S852.65[农业科学—基础兽医学]