山羊痘病毒疫苗株和野毒株ORF121基因比较分析  

Comparation analysis of ORF121 gene from goat poxvirus vaccine strain and wild strain

作　　者：赵俊皓 张克山[2] 卢炳州 王文芳[1,2] 郑海学[2] 刘湘涛[2] 胡永浩[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医学报》2017年第4期648-652,共5页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2015BAD12B04)

摘　　要：山羊痘(goat pox,GP)是由山羊痘病毒(goat poxvirus,GPV)感染引起山羊的一种烈性传染病,弱毒疫苗接种是预防和控制GP的重要手段之一。ORF121是GPV编码和GPV转录、复制及细胞内包装相关的基因之一,在GPV突破宿主细胞免疫系统过程中扮演重要角色。为比较分析GPV疫苗弱毒株和野毒株编码的ORF121基因特征,本研究扩增并测定了GPV疫苗株和野毒株的ORF121基因序列,比较分析了二者核酸水平和氨基酸水平的变异及其蛋白质二、三级结构差异。结果表明,GPV疫苗弱毒株和野毒株编码的ORF121基因核苷酸序列相似性为97.9%,氨基酸序列相似性为98.2%。本研究结果为揭示GPV异原宿主致弱的机制积累了资料。Goat pox is an virulent infectious disease caused by goat poxvirus（GPV）. Attenuated vac- cination is one of the important measures to prevent and control GP. ORF 121 is encoded by GPV and related to transcription,replication and virus packaging in vivo. ORF121 gene plays an important role in GPV breakthrough host cellular immune system. In order to analyze the different characters between ORF121 genes from GPV wild strain and attenuated vaccine strain, we amplified and sequenced nucleotide sequence of the two GPV genes,compared the nucleotide sequences and amino acid sequences, and predicted the secondary and tertiary structure. The results show that ORF121 gene from GPV wild strain and vaccine strain shared 97.90/oo identity at nucleotide level and 98.2% identity at amino acid level. This result contributes to exploring the mechanism of GPV attenuated.

关 键 词：山羊痘病毒 ORF121基因 疫苗株 野毒株 

分 类 号：S852.65[农业科学—基础兽医学] R535[农业科学—兽医学]