犬吉氏巴贝斯虫人工感染试验方法的建立  

Methods of Babesia gibsoni artificial infection

作　　者：杨伦[1] 闫可可[1] 钱萌希 许家荣[1] 杨德吉[1] 姚大伟[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《中国兽医学报》2017年第4期668-670,共3页Chinese Journal of Veterinary Science

基　　金：南京农业大学SRT计划基金资助项目(1417A04);南京农业大学动物医学院教学改革资助项目(2014-2015)

摘　　要：选用健康本地杂种犬,分为2组,一组手术切除脾脏,另一组未经任何处理,然后2组犬人工静脉接种含犬吉氏巴贝斯虫的病犬血液,接种后观察临床症状、进行实验室检查(血涂片、血常规、病原的PCR检测),检测2组犬人工感染犬吉氏巴贝斯虫后的发病情况。结果显示,人工感染后2组犬均表现出自然感染犬吉氏巴贝斯虫后的临床症状,实验室检查血涂片中检测到犬吉氏巴贝斯虫虫体,血常规表现出严重的贫血,PCR能扩增出犬吉氏巴贝斯虫的基因片段。2组犬比较显示,切脾组的红细胞感染率和贫血程度均高于未切脾犬。本试验成功建立了犬吉氏巴贝斯虫人工感染方法,且脾切除后感染效果优于直接静脉感染。The aim of this research was to explore methods of Babesia gibsoni artificial infection and to establish the basis of studying on canine babesiosis. Six heath domestic dogs were divided into two groups. Three dogs were carried out splenectomy and 3 dogs were no treated as control. All dogs were administered sickness dog blood which was infected by Babesia gibsoni. Clinical symptoms,laboratory examination（such as blood smear,complete blood count,PCR diagnosis of pathogen） were detected after infection. The results showed that the dogs infected with Babesia gibsoni by artificial method displayed the simile clinical symptoms with natural infected dogs. Babesia gib- soni could be found in blood smear. The dogs infected with B. gibsoni showed serious anemia ac- cording to results of complete blood count. The gene fragment of Babesia gibsoni could be detected in infected dogs using PCR method. Infection rate of red blood and degree of anemia in the splenee- tomy group were higher than control groups. It was suggested that the methods of Babesia gibsoni artificial infection was established successfully in this research. Splenectomy before infection was the better choice for Babesia gibsoni artificial infection.

关 键 词：犬 巴贝斯虫 脾切除 

分 类 号：S855.9[农业科学—临床兽医学] S852.723[农业科学—兽医学]