玉米杂交种子纯度的SSR分子标记与盐溶蛋白检测法的比较研究  

The Comparison Research of SSR Molecular Marker And Salt-Soluble Protein PAGE In Identification of Maize Seed Purity

作　　者：王晓梅[1] 傅迎军[1] 王燕平[1] 王晓东[1] 孙殷会[1] 张庆娜[1] 白艳凤[1] 徐德海[1] 温义昌[2] 李贵全[2] 索修改 

机构地区：[1]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041 [2]山西农业大学研究生学院,山西太谷030801

出　　处：《中国林副特产》2017年第2期4-8,共5页Forest By-product and Speciality in China

基　　金：国家科技支撑计划项目(2014BAD01B01);牡丹江市科学技术计划项目(Z2015n0011)

摘　　要：以我国推广面积较大的9个玉米杂交种为试材,利用盐溶蛋白电泳法与SSR分子标记鉴定法对试验材料进行了杂交种纯度鉴定的比较试验。结果表明:盐溶蛋白电泳法可以很好地鉴定出8个组合,且具有简单、快速、准确、稳定和低成本的特点,可用于大批量检测。但因一些种子蛋白质水平差异较小,有1个组合难以鉴定,具有一定的局限性。而SSR分子标记法经过40对引物的重复筛选,仅用3个多态性好的SSR引物能鉴定出所有的组合,具有更好的精确性。但因实验条件要求高,成本相对较高,不宜用于大批量检测。因此,可将盐溶蛋白电泳法作为首选大批量种子纯度的检测方法,而对难以鉴定的品种可采用SSR分子标记法进行鉴定。In this study, Took 9 maize hybrids with larger area as material, salt-soluble protein PAGE and SSR analysis were used in Comparing test of hybrids purity. The results showed that 8 hybrid could be distinguished by salt- soluble protein PAGE, and it was simple ,quick,accurate, stable and cheap characteristics and can be applied in a large scale. One combinations won, t identified by salt-soluble protein PAGE because of the limitation of the low variation among salt-soluble proteins. 40 primerswere used in SSR analysis and only 3 primer combinations could identify all the 9 hybrid corn combinations, But it canct be applied in a large scale on account of its high expense. In con- clusion, salt soluble protein PAGE can be used in most cases and SSR can be used in identifying difficultly.

关 键 词：种子纯度 盐溶蛋白电泳 SSR分子标记 

分 类 号：S513[农业科学—作物学]