A抗原减弱表型的基因分析  

Gene analysis of A antigen weakened phenotype

作　　者：马晓莉[1] 马宏伟[1] 金新莉[2] 杨贺才[3] 陈赞[1] 

机构地区：[1]河南省红十字血液中心血型室,郑州450000 [2]河南省红十字血液中心检验科,郑州450000 [3]河南省红十字血液中心科研外事科,郑州450000

出　　处：《郑州大学学报（医学版）》2017年第2期191-193,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关项目201503199

摘　　要：目的:研究A抗原减弱的分子遗传学背景。方法:用血清学的方法筛选出2015年1月至11月A抗原减弱的样本6例,对其ABO基因的第6、7外显子进行测序分析。结果:6例中基因型为A102/O01有2例,A102/O02有1例,A102/B101有2例,1例样本发现新的等位基因。结论:由于ABO基因的多态性,除了常见的第6、7外显子上的基因突变引起抗原减弱外,第6、7外显子以外的基因变异或转录结构以及调控区域的异常都有可能导致A抗原减弱。Aim： To study the molecular genetic background of A antigen weakening by serologically detecting the A antigen weakened phenotype samples and ABO gene sequencing. Methods： Serological method was used to screen A antigen weakened samples blood donors of from during January 2015 to November 2015. The exons 6 and 7 of the ABO gene were amplified by PCR and sequenced. Results： There were 6 cases with A-antigen-attenuated type and their genotypes were A102 / O01（ 2 cases）,A102 / O02（ 1 case）,A102 / B101（ 2 cases）,and a new allele（ 1 case）. Conclusion： Because of the ABO gene polymorphism,in addition to the the mutations in exons 6 and 7,other gene mutation or transcriptional structure as well as abnormalities of regulation region are likely to lead to A antigen weakening.

关 键 词：抗原减弱 ABO血型 基因分析 

分 类 号：R392.7[医药卫生—免疫学]