代谢综合征患者痰、郁证素与外周血miRNAs表达相关性临床研究  

Clinical Study of Correlation between Syndrome Elements of Phlegm and Qi Stagnation and miRNAs Expression of Peripheral Blood in Metabolic Syndrome

作　　者：丁珊珊[1] 林敏[1] 张凌媛[1] 康洁[1] 陈淑娇[1] 

机构地区：[1]福建中医药大学中医证研究基地,福建福州350122

出　　处：《山东中医药大学学报》2017年第2期134-137,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:.81503498);福建省自然科学基金项目(编号:2016J01780);福建中医药大学重点学科校管课题(编号:X2014090-学科)

摘　　要：目的:探讨代谢综合征痰、郁证素与外周血miRNAs表达的相关性。方法:选择40~55岁女性代谢综合征患者,采用证素辨证并根据证素积分分为郁证组(痰积分≥100分、气滞积分≥70分,n=19)和非郁证组(痰积分≥100分、气滞积分<70分,n=22),同时纳入40~55岁女性健康体检者为对照组(n=20)。采用Real-time PCR法对所有研究对象的外周血miRNAs(miR-15b-5p、miR-142-3p、miR-221-3p和miR-451a)表达进行检测。结果:与对照组比较,郁证组miR-15b-5p表达下降(P<0.01),miR-142-3p、miR-221-3p(P<0.05)、miR-451a(P<0.01)表达上升;非郁证组miR-15b-5p(P<0.01)、miR-221-3p(P<0.01)表达下降,miR-142-3p(P<0.01)、miR-451a表达上升。与非郁证组比较,郁证组miR-15b-5p(P<0.05)、miR-221-3p(P<0.01)、miR-451a(P<0.01)表达上升。结论:40~55岁女性代谢综合征患者痰、郁证素与外周血miR-15b-5p、miR-142-3p、miR-221-3p和miR-451a的表达存在一定的相关性。Objective：To explore the correlation between syndrome elements of phlegm and qi stagnation and miRNAs expression of peripheral blood in metabolic syndrome.Methods：The syndrome element differentiation was conducted based on diagnosis informations collected from female patients aged 40 to 55 with metabolic syndrome.The qi stagnation group consisted of 19 patients with the accumulated phlegm score larger than 100 and qi stagnation score larger than 70,while the non-qi stagnation group consisted of 22 people with the accumulated phlegm score larger than 100 and qi stagnation score less than 70.Total 20 healthy women were included as the control group.The miRNAs（miR-15b-5p、miR-142-3p、miR-221-3p and miR-451a） expressions of peripheral blood were detected by the method of real-time PCR.Results：Compared with the control group,the miR-15b-5p expressions were reduced（P〈0.01）,miR-142-3p,miR-221-3p（P〈0.05）,miR-451a（P〈0.01） were increased in the qi stagnation group;the expression of miR-15b-5p（P〈0.01）,miR-221-3p（P〈0.01） were reduced,miR-142-3p（P〈0.01）,miR-451 a were increased in the non-qi stagnation group.The expressions of miR-15b-5p（P〈0.05）、miR-221-3p（P〈0.01） and miR-451a（P〈0.01） of the depression group were higher than the non-qi stagnation group.Conclusion：Some correlations between syndrome elements of phlegm and qi stagnation and miRNAs expression of peripheral blood exist in female patients aged 40 to 55 with metabolic syndrome.

关 键 词：代谢综合征 痰 郁 证素 MIRNAS 

分 类 号：R589[医药卫生—内分泌]