响应面法优化益智醒脑颗粒水提工艺  

Optimization of water extraction technique for Yizhi Xingnao granules through response surface methodology

作　　者：陈冬冬[1,2] 张焱[1,2] 程建明[1,2] 钱海峰[1,2] 

机构地区：[1]南京中医药大学药学院,南京210000 [2]江苏省中药功效物质重点实验室,南京210000

出　　处：《中国新药杂志》2017年第7期813-817,共5页Chinese Journal of New Drugs

基　　金：江苏高校优势学科建设工程资助项目

摘　　要：目的:对益智醒脑颗粒的水提工艺进行筛选,优选出最佳水提工艺。方法:采用HPLC方法测定二苯乙烯苷,通过加水量和提取时间的合理分配并结合Box-Behnken响应面法,以加水量、提取时间、提取次数为自变量,二苯乙烯苷提取率、浸膏得率为因变量,优化益智醒脑颗粒水提工艺。结果:最佳提取工艺为加18倍量水(8+5+5),加热回流提取3次,提取3.5 h(1.5+1+1)。结论:该提取工艺稳定、可靠。Objective： To optimize water extraction technology for screening the water extraction process of Yizhi Xingnao granule. Methods： The stilbene glucosides were determined by HPLC. Taking extraction rate of stilbene glucoside and extract yield as indexed,water addition,extraction time and extraction times were researched through the reasonable allocation of water addition,extraction time and response surface methodology. Results：The best extracting technology is using the amount of 18（ 8 ＋ 5 ＋ 5） times water,extracting 3 times and extracting for 3. 5 h（ 1. 5 ＋ 1 ＋ 1）. Conclusion： This extraction technology is stable and reliable.

关 键 词：益智醒脑颗粒 响应面优化 二苯乙烯苷 

分 类 号：R284.2[医药卫生—中药学]