富氢液对大鼠肾缺血再灌注肾功能及TLR4/NF-κB信号通路表达的影响  

Effect of hydrogen rich saline on renal function and expression of TLR4/NF-kappa B signal pathway in rats with renal ischemia-reperfusion injury

作　　者：陈克研[1] 董婉维[1] 

机构地区：[1]中国医科大学实验动物部,辽宁沈阳110122

出　　处：《解剖科学进展》2017年第2期174-176,180,共4页Progress of Anatomical Sciences

基　　金：国家自然科学青年基金(31201758);中国医科大学新教师基金项目(XZR20160036)

摘　　要：目的探讨富氢液(HRS)对大鼠肾缺血再灌注肾功能及TLR4/NF-κB信号通路表达的影响。方法SPF级雄性SD大鼠30只,随机分为假手术组(S组)、缺血再灌注组(I/R组)、富氢液组(HRS组),每组10只。S组麻醉开腹后右肾切除;I/R组行右肾切除,左肾缺血45min,再灌注60min,造肾缺血再灌注模型;HRS组于术前口服40 ml/kg富氢液,连续3d后建立I/R模型。每组于I/R后6h取取血样,分离血浆,多功能生化仪检测肌酐(Scr)和尿素氮(BUN)含量;ELISA检测IL-1β和TNF-α含量;另取肾脏,HE染色观察病理组织学变化,Western-blot检测TLR4、Myd88、NF-κB表达变化。结果 HRS可减轻由I/R造成的肾损伤,减低血浆中Scr、BUN、IL-1β和TNF-α含量,抑制肾脏TLR4、Myd88、NF-κB表达(P<0.05)。结论 HRS减轻由I/R引起的肾功能障碍,抑制炎性反应,对肾脏有一定的保护作用,其机制可能与TLR4/NF-κB信号通路有关。Objective To explore the effect of hydrogen rich saline（HRS） on renal function and expression of TLR4/NF-kappa B signal pathway in rats with renal ischemia-reperfusion injury. Methods SPF SD rats（n=30） were randomly divided into sham group（S）, ischemia reperfusion（I/R group） and HRS（40 ml/kg） group averagely.The serum creatinine（Scr） and urea nitrogen（BUN） contents were determined, contents of IL-1-αand TNF-αwere detected by ELISA, kidney pathological changes were observed with HE staining, expression of Myd88, NF-kappa B and TLR4 determined by Western-blot. Results HRS reduced the renal damage caused by I/R, reduced the contents of Scr, BUN, IL-1 and TNF-α in plasma, inhibited the expressions of TLR4, Myd88 and NF-kappa B in kidney（P〈0.05）. Conclusion The protective effect of HRS on the I/R kidney might be related to the TLR4/NF-κB signalpathway.

关 键 词：富氢液 肾脏 缺血再灌注 TLR样受体4 核转录因子kappa B 

分 类 号：R691.6[医药卫生—泌尿科学]