miR-34a调控HMGB1表达对人膀胱癌细胞增殖的影响  

The effect of miR-34a on the proliferation of human bladder cancer cells by directly repressing HMGB1

作　　者：苏允伟 祝兴旺[1] 杨玉彬[1] 刘锁民[1] 薛东炜[1] 刘屹立[1] 

机构地区：[1]中国医科大学第四附属医院泌尿外科,辽宁沈阳110032

出　　处：《解剖科学进展》2017年第2期181-184,共4页Progress of Anatomical Sciences

基　　金：辽宁省科学计划项目(2015225009)

摘　　要：目的探讨miR-34a对膀胱癌T24细胞增殖的影响。方法合成miR-34a的模拟物并转染膀胱癌T24细胞,荧光显微镜下观察转染效率,实时荧光定量PCR法检测转染48 h后,T24细胞中miR-34a的表达情况,MTT法检测转染24、48和72 h后T24细胞的增殖情况,实时荧光定量PCR法和western blot方法检测转染48 h后膀胱癌细胞内源性HMGB1 m RNA及蛋白的表达变化。结果 miR-34a的模拟物转染至T24细胞的效率为80%,转染miR-34a模拟物48 h后,T24细胞内miR-34a的表达显著升高(P<0.05),T24细胞的增殖率显著低于阴性对照组(P<0.05),且呈时间依赖性,HMGB1 m RNA及蛋白的表达水平明显低于阴性对照组(P<0.05)。结论 miR-34a抑制膀胱癌T24细胞增殖可能与靶向HMGB1基因相关。Objective To investigate the effect of miR-34 a on the proliferation of human bladder cancer cells by directly repressing HMGB1. Methods miR-34 a mimics and miR-34 a mimics negative control were synthesized and transfected into bladder cancer T24 cells. The transfection efficiency was observed under a fluorescence microscope after miR-34 a mimics was transfected. The expression level of miR-34 a in T24 cells was detected by real-time PCR at 48 h after transfection, the proliferation of T24 cells was detected by MTT assay. The expression of HMGB1 mRNA and protein in T24 cells was detected by real-time PCR and Western blot methods respectively after miR-34 a mimics was transfected. Results The efficiency of miR-34 a mimics transfection into T24 cells was 80%, the expression level of miR-34 a in T24 cells was higher（P〈0.05）, but the proliferative rates of T24 cells were lower in miR-34 a mimics transfection than in the negative control group（P〈0.05）with a timedependent manner. The expression level of HMGB1 mRNA and protein in T24 cells was lower in miR-34 a mimics transfection group than in the negative control group（P〈0.05）. Conclusion The inhibition of Mi R-34 a on the proliferation of T24 cells might be related to repressing HMGB1 gene.

关 键 词：MIR-34A 膀胱癌 HMGB1 增殖 T24细胞 

分 类 号：Q737.14[生物学—分子生物学]