携带凝血酶原复合物的阴离子脂膜微泡造影剂联合超声治疗对兔肾创伤性出血模型的止血效果  

Prothrombin complex concentrate carrying anionic lipid-coated microbubbles enhanced therapeutic ultrasound in hemostatic treatment for renal trauma in rabbits models

作　　者：乔伟[1] 杨莉[1] 钱胜利[1] 李延[1] 张俊[1] 周木秀[1] 刘政[2] 

机构地区：[1]中国人民解放军第161医院特诊科,湖北武汉430010 [2]第三军医大学新桥医院超声科,重庆400037

出　　处：《中国医学影像技术》2017年第4期494-498,共5页Chinese Journal of Medical Imaging Technology

基　　金：2015-2016年度湖北省卫生计生指导性项目(WJ2015Z036)

摘　　要：目的探讨携带凝血酶原复合物的阴离子脂膜微泡(PCCMB)造影剂联合超声治疗对兔肾创伤性出血模型的止血效果。方法对24只健康新西兰大白兔建立兔肾创伤性出血模型,并随机分为3组,即单纯超声治疗组(US组)、单纯PCCMB注射组(SHAM组)及PCCMB注射联合超声治疗组(PCCMB+US组),每组各8只。对各组均通过10min出血量及视觉评分评价止血效果。并对SHAM组及PCCMB+US组于超声治疗前和治疗后即刻、治疗后60min行CEUS检查,对US组于超声治疗后即刻及治疗后60min行CEUS检查,测量兔肾造影峰值强度(PI)。结果所有实验兔均顺利完成实验。治疗后PCCMB+US组10min出血量低于SHAM组及US组(P均<0.05);PCCMB+US组视觉评分明显低于SHAM组及US组(P均<0.05)。PCCMB+US组超声治疗后即刻及治疗后60min的PI均高于超声治疗前(P均<0.05)。SHAM组及US组超声治疗前与治疗后即刻及治疗后60min PI差异均无统计学意义(P均>0.05)。结论PCCMB造影剂联合超声治疗对兔肾脏创伤性出血可起到快速、有效止血的效果。Objective To investigate the hemostatic effects of prothrombin complex concentrate carrying anionic lipid coated microbubbles （PCCMB） enhanced therapeutic ultrasound for renal trauma in rabbits models. Methods Twenty-four healthy New Zealand rabbits were randomly divided into three groups （each n=8）, including simple therapeutic ultrasound group （US group）, simple PCCMB injection group （SHAM group） and PCCMB injection combined with therapeutic ultrasound group （PCCMB＋US group）. Visual bleeding score and 10-min bleeding volume were evaluated for hemostatic efficacy. CEUS was used to assess the kidney perfusion in SHAM and PCCMB＋US groups before therapeutic ultrasound, immediately and 60 min after therapeutic ultrasound. And CEUS was performed on US group 60 min after therapeutic ultrasound. The acoustic peak intensity （PI） of kidney in rabbit was measured. Results The treatment was successfully completed in all the experimental rabbits. The bleeding scores and the 10-min hemorrhagic volumes decreased significantly in PCCMB＋US group compared with the other two groups （both P〈0.05）. After therapeutic ultrasound, visual bleeding score of PCCMB＋US group was respectively lower than that of US group and SHAM group （both P〈0.05）. In PCCMB＋US group, PI obtained immediately and 60 min after therapeutic ultrasound were higher than that obtained before therapeutic ultrasound （both P〈0.05）. There was no significant difference of PI before and after therapeutic ultrasound in US and SHAM groups （all P〉0.05）. Conclusion PCCMB enhanced therapeutic ultrasound provides an effective way for renal trauma in rabbits.

关 键 词：超声疗法 造影剂 肾创伤 止血 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]