Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology  

作　　者：杨慧琴 陈天翔 刘琳 陈德强 李树白 权静 朱利民 聂华丽 

机构地区：[1]Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China [2]College of Chemistry, Chemical Engineering and Biotechnology , Donghua University, Shanghai 201620, China [3]Changzhou Institute of Engineering Technology, Changzhou 213164, China

出　　处：《Journal of Donghua University(English Edition)》2017年第1期111-117,共7页东华大学学报（英文版）

基　　金：Fundamental Research Funds for the Central Universities,China(No.2232015D3-15);Shanghai Natural Science Foundation,China(No.14ZR1401300);Shanghai Pujiang Program,China(No.16PJD007);“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)

摘　　要：A new cross-linked heptapeptide matrix(CHM)was prepared and the adsorption performance for papain was investigated.Firstly,a phage-displayed heptapeptide(Ile-Gln-SerPro-His-Phe-Phe)with high affinity to papain was found.Secondly,the heptapeptide was cross-linked to a matrix for papain adsorption.Finally,the adsorption conditions were optimized by response surface methodology.The results indicated that the optimum adsorptionconditionsweredeterminedasinitialpH 6.9,temperature 40℃,and the adsorption capacity of CHM for papain was found to be 55.52 mg/g.Moreover,the papain was purified 78-fold in a single step determined by affinity precipitation.More than87%of the adsorbed papain was desorbed using the eluent solventtriethylamine at pH 7.4.The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adsorption. Finally, the adsorption conditions were optimized by response surface methodology. The results indicated that the optimum adsorption conditions were determined as initial pH 6.9, temperature 40℃, and the adsorption capacity of CHM for papain was found to be 55.52 mg/g. Moreover, the papain was purified 78- fold in a single step determined by affinity precipitation. More than 87 % of the adsorbed papain was desorbed using the eluent solventtriethylarnine at pH 7.4. The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.

关 键 词：heptapeptide PAPAIN response surface methodology ADSORPTION affinity precipitation 

分 类 号：Q184.1[生物学—普通生物学]