葡聚糖硫酸钠自由饮用与灌胃诱导小鼠溃疡性结肠炎模型的对比研究  

Comparison of Ulcerative Colitis Models Respectively Induced by Free Drinking and Intragastric Administration of Dextran Sodium Sulfate in Mice

作　　者：衡宇 李晰[1] 孙涛[1] 张琰[1] 杨鹏[1] 

机构地区：[1]第四军医大学唐都医院药剂科,西安710038

出　　处：《中国药师》2017年第4期603-606,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:81403170)

摘　　要：目的:比较葡聚糖硫酸钠(DSS)自由饮用与灌胃两种给药方式诱导小鼠溃疡性结肠炎(UC)疾病相关指标的差异,为优化UC小鼠造模方法提供实验参考。方法:将C57BL/6小鼠随机分为正常对照组、自由饮用组和灌胃组3组,每组10只,分别自由饮水、自由饮用3%DSS溶液及4 g·kg^(-1)·d^(-1)灌胃DSS,连续7 d,建立UC模型。以疾病活动指数(DAI)、小鼠结肠的组织学损伤评分及髓过氧化物酶(MPO)活性为评价指标,比较两种给药方式的各指标均数及变异系数的差异。结果:自由饮用组造模过程中有2只小鼠死亡,造模结束后存活小鼠的DAI值为(8.8±1.6)分,灌胃组无小鼠死亡,其DAI值为(9.0±0.8)分,两组间差异无统计学意义(P>0.01),但自由饮用组的变异系数高于灌胃组(18.7 vs.8.6)。自由饮用组与灌胃组结肠组织学损伤评分分别为(24.8±4.2),(27.0±2.8)分,均呈典型性炎症改变,两组间差异无统计学意义(P>0.05),但自由饮用组的变异系数高于灌胃组(16.9 vs.10.4)。正常对照组、自由饮用组与灌胃组结肠组织MPO分别为(0.41±0.03),(2.32±0.34),(2.05±0.18)U·g^(-1),与正常对照组相比,自由饮用组与灌胃组的MPO差异有统计学意义(P<0.01),自由饮用组与灌胃组两组间MPO差异无统计学意义(P>0.05),但自由饮用组的变异系数高于灌胃组(14.7 vs.8.8)。结论:自由饮用组与灌胃组均可成功诱导小鼠UC模型。与自由饮用组相比,灌胃组各项指标数据的变异系数小,离散度低,采用灌胃法制备溃疡性结肠炎小鼠模型,动物表现更均一。Objective： To investigate the differences in related indices of ulcerative colitis （UC） respectively induced by free drinking and intragastric administration of dextran sodium sulfate （DSS） in mice to provide experimental reference for the optimization of UC model. Methods： Totally 30 C57BL/6 mice were randomly divided into the normal control group, free drinking group and intragastric administration group with 10 ones in each. The mice drank water freely with free drinking or intragastric administration of 3% DSS solution at the dose of 4 g ·kg^-1· day^-1 for 7 days to establish the UC model. The differences in disease activity index （DAI）, histological damage sore and activity of myeloperoxidase （MPO） among the groups were compared. Results： Two mice died during the experiment in the free drinking group, and DAI of survival mice was （8.8 ± 1.6）. There was no death of mice in intragastric administration group, and DAI was （9.0±0.8）, and there was no significant difference in DAI between the groups （ P 〉 0.05 ）, while the coefficient of variation in the free drinking group was higher than that in the intragastric administration group （18.7 vs 8.6）. The colonic histological damage score of the free drinking group and the intragastric administration group was 24.8 ± 4.2 and 27.0 ± 2.8, respectively, which was typical inflammatory change with no significant difference （ P 〉 0.05 ）, while the coefficient of variation of the free drinking group was higher than that of the intragastric administration group （ 16.9 vs 10.4）. MPO of the normal control group, free drinking group and intragastric administration group was （0.41 ± 0.03）, （ 2.32 ± 0.34） and （2.05 ± 0.18 ） U · g^ - 1, respectively. Compared with the normal control group, significant difference in MPO was shown in the free drinking group and the intragastric admin- istration group （P 〈 0.01 ）, while there was no significant difference in MPO between the groups （P 〉 0.05 ）, and th

关 键 词：溃疡性结肠炎 葡聚糖硫酸钠 造模方法 自由饮用 灌胃 

分 类 号：R965.2[医药卫生—药理学]