毓麟合剂对肾阳虚型排卵障碍大鼠卵巢功能的影响研究  

Effect of Yulin Mixture on Ovarian Function in Rats with Kidney-yang Deficiency Ovulatory Dysfunction

作　　者：张玉萱[1] 徐玲玲[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院药剂科,上海200437

出　　处：《中国药师》2017年第4期621-623,共3页China Pharmacist

基　　金：上海市进一步加快中医药事业发展三年行动计划(2014-2016年)建设项目(编号:ZY3-JSFC-2-2019)

摘　　要：目的:观察毓麟合剂对氢化可的松致排卵障碍大鼠相关脏器指数、卵巢形态和性激素的影响。方法:建立氢化可的松致大鼠排卵障碍模型,分为模型对照组、毓麟合剂组、阳性对照组(枸橼酸氯米芬组),另设正常对照组,每组10只。观察大鼠一般情况,用电子分析天平测子宫、卵巢湿质量指数,采用放射免疫分析法检测血清雌二醇(E_2)、孕酮(P)、卵泡刺激素(FSH)、黄体生成素(LH);HE染色后观察卵巢形态。结果:毓麟合剂可改善排卵障碍大鼠一般状况,升高其子宫、卵巢指数(P<0.05),升高血清E_2、P、LH、FSH水平(P<0.01),改善病理状态下卵巢的形态结构。结论:提示毓麟合剂可通过提高血清E2、P、LH、FSH水平,从而促进卵巢发育。Objective： To observe the effect of Yulin mixture on ovarian function in rats with kidney-yang deficiency ovulatory dysfunction. Methods： The hydrocortisone-induced ovulation disorder rat models were established, and the rats were divided into the model control group, Yulin mixture group and positive control group （clomiphene citrate group）, and the normal control group was set up, and 10 rats were in each group. The general situation of the rats was observed. The wet weight indices of uterus and ovary were measured by an electronic analytical balance. Serum estradiol （E2） , progesterone （P）, follicle stimulating hormone （FSH） and luteinizing hormone （LH） were measured by radioimmunoassay. Ovarian morphology was observed after HE staining. Results： Yulin mixture could improve the general status of ovulation disorder rats, increase the wet weight indices of uterus and ovary （ P 〈 0.05 ） and the levels of serum E2, P, LH and FSH （P 〈 0.01 ）, and improve the pathological state of ovarian morphology. Conclusion： Yulin mixture can improve the levels of serum E2, P, LH and FSH resulting in promoting ovarian development.

关 键 词：毓麟合剂 排卵障碍 性激素 卵巢形态 

分 类 号：R285.5[医药卫生—中药学]