参芪利心胶囊的提取工艺研究  

Study on the Extraction Technology of Shenqi Lixin Capsules

作　　者：吴伦[1] 苏阳[1] 陈晓宇[1] 田振坤[1] 谢宁[2] 刘莉[2] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第一医院

出　　处：《中国药师》2017年第4期624-628,共5页China Pharmacist

基　　金：教育部高等学校博士学科点专项科研基金(编号:20102327110002);黑龙江省科技攻关计划项目(编号:GC09C404)

摘　　要：目的:通过药效学评价结合正交试验多指标综合评分法优选参芪利心胶囊最佳提取工艺。方法:采用腹腔注射多柔比星法建立充血性心力衰竭(CHF)模型,以CHF大鼠左室收缩末期内径(LVEDD)、左室舒张末期内径(LVESD)、短轴缩短率(FS)和左室射血分数(LVEF)为指标,筛选样品提取工艺(A工艺为药材全煎煮;B工艺为太子参细粉与其他药材煎煮后混合)。通过单因素考察并设计正交试验,以黄芪甲苷、丹参酮Ⅱ_A含量和煎煮物重量为指标,考察加水量、煎煮次数、煎煮时间对提取工艺的影响并对其进行优化。结果:药效学实验表明B工艺样品对CHF大鼠心脏症状及心功能改善作用更佳。该工艺中其他药材最优提取条件为每次加12倍量水煎煮1 h,共煎煮3次。验证试验结果显示黄芪甲苷、丹参酮ⅡA的平均提取率分别为61.82%、50.07%,平均煎煮物重量为6.02 g。结论:参芪利心胶囊最优的提取工艺科学合理、稳定可靠。Objective： To optimize the extraction process of Shenqi Lixin capsules through pharmaeodynamic evaluation combined with orthogonal experiments with multi-index comprehensive evaluation. Methods： The congestive heart failure（CHF） model was established by intraperitoneal injection of doxorubicin in rats, and the LVEDD, LVESD, FS and LVEF of CHF rats were used as the indicators to screen the extraction process of the samples （ process A was with the whole decoction of herbs and process B was with heterophylla powder mixed with the other herbs after boiling）. The single factor tests and orthogonal tests were used to optimize the extraction process by taking the contents of astragaloside and tanshinone ⅡA and the quality of the decocted material as the indices, and adding water amount, decocting times and duration as the influencing factors. Results： Pharmacodynamic experiments indicated that the improvement effects of the samples from process B on cardiac symptoms and cardiac function in CHF rats were better than that of the samples from process A. The other medicinal materials were decocted by 12-fold amount of adding water, and repeated for 12 times with one hour for each time. The average extraction rate of astragaloside and tanshinone ⅡA was 61. 82% and 50.07%, respectively, which was proven by the verification experiments. The average weight of the decoction was 6.02 g. Conclusion： The optimized extraction process of Shenq Lixin capsules is scientific, reasonable, stable and reliable.

关 键 词：参芪利心胶囊 心功能 提取工艺 正交试验 黄芪甲苷 丹参酮ⅡA 

分 类 号：R284.2[医药卫生—中药学]