HPLC法测定姜黄素-槲皮素复方自微乳的载药量和包封率  

Determination of the Entrapment Efficiency and Drug Loading Capacity of Curcumin and Quercetin Loaded Self-microemulsifying Drug Delivery System

作　　者：黄瑞雪[1] 黎翊君 茅玉炜 刘乐环 王渐鸿 余家齐 戴俊东[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国药师》2017年第4期664-667,共4页China Pharmacist

基　　金：北京中医药大学自主选题项目(编号:2016-JYB-XS072)

摘　　要：目的:建立HPLC法测定姜黄素-槲皮素复方自微乳(CUR-QUE-SMEDDS)的载药量和包封率。方法:采用离心法分离游离药物,HPLC法测定药物含量。色谱柱:Purospher STAR LP C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-4%冰醋酸(50∶50),流速:1.0 ml·min^(-1),检测波长:370 nm,柱温:30℃,进样量:10μl。结果:姜黄素和槲皮素的线性范围分别为10.728~96.552μg·ml^(-1)(r=0.999 8)和1.08~9.72μg·ml^(-1)(r=0.999 9),平均回收率分别为99.98%(RSD=1.46%,n=9)和100.34%(RSD=1.06%,n=9)。CUR-QUE-SMEDDS中姜黄素和槲皮素的包封率分别为(95.97#0.50)%和(95.91#2.52)%,载药量分别为(25.82#0.15)mg·g^(-1)和(1.80#0.05)mg·g^(-1)。结论:该法准确可靠,快速简便,适用于测定CURQUE-SMEDDS的载药量和包封率。Objective： To establish an HPLC method to determine the entrapment efficiency （EE） and drug loading （DL） of curcumin （CUR）and quereetin （QUE）loaded self-mieroemulsifying drug delivery system. Methods： A centrifugation method was used to isolate the free drug. The eontent of drug was determined by HPLC. The analytical column was a Purospher STAR LP C18 column （250 mm×4.6 mm, 5 μm） and the column temperature was 30℃. The mobile phase was acetonitrile-4% acetic acid （50：50） and the flow rate was 1.0 ml· min^-1. The UV detection wavelength was set at 370 nm and the injection volume was 10μl. Results： CUR and QUE were linear within the range of 10.728-96. 552μg·ml^- 1（ r = 0.999 8 ） and 1.08-9.72 μg · ml^ - 1 （ r = 0. 999 9 ） , respectively. The average recovery was 99.98% （ RSD = 1.46% , n = 9 ） and 100.34% （ RSD = 1.06% , n = 9 ）, respectively. In CUR-QUESMEDDS, the EE of curcumin and quercetin was （95.97 · 0.50） % and （95.91 ·2.52） %, and the DL was （25.82·0.15） mg · g^ - 1 and （1.80±0.05） mg · g^-1, respectively. Conclusion： The method is accurate, rapid and simple, and suitable for the determination of DL and EE in CUR-QUE-SMEDDS.

关 键 词：姜黄素 槲皮素 复方自微乳 高效液相色谱法 载药量 包封率 

分 类 号：R927.2[医药卫生—药学]