基于羟基自由基高级氧化快速杀灭锥状斯氏藻孢囊的研究  

Inactivation of Scrippsiella trochoidea cysts using hydroxyl radials

作　　者：郭枫[1] 李超[2] 白敏冬[1] 林艳强 贺霄[1] 郑琦琳 

机构地区：[1]厦门大学福建省海洋生物资源开发利用协同创新中心滨海湿地生态系统教育部重点实验室环境与生态学院,福建厦门361102 [2]厦门大学海洋与地球学院,福建厦门361102

出　　处：《海洋学报》2017年第4期101-106,共6页

基　　金：国家科技支撑计划项目(2013BAC06B01;2013BAC06B02);国家重大科研仪器研制项目(61427804);科技部创新人才推进计划重点领域创新团队(2015RA4008)

摘　　要：随着船舶压载水的转运,我国面临严重的外来入侵生物风险,其中部分赤潮藻形成的孢囊可成为赤潮的"种源",严重危害近岸海洋环境,因而快速有效地杀灭赤潮藻孢囊至关重要。本文利用大气压强电离放电高效生成的羟基自由基(·OH),对典型赤潮藻锥状斯氏藻孢囊进行杀灭研究。采用萌发实验、SYTOX Green荧光染色法确定·OH杀灭锥状斯氏藻孢囊的阈值和时间,扫描电子显微镜观察孢囊的形态变化。结果表明,·OH杀灭孢囊的CT阈值为0.49mg·min/L,时间为10s,·OH氧化降解孢囊体内叶绿素,破坏DNA,抑制萌发,具有其他方法无法比拟的优势。因此,·OH快速杀灭赤潮藻孢囊的新方法,对防控外来入侵生物引发的海洋赤潮灾害具有重要的作用。Resting cysts are largely formed during the red tide which may be the ＂seed hank＂ of next harmful algal bloom. And the resting cysts are resistant to normal physical and chemical treatments due to their thick cyst walls. In this paper, the resting cysts of Scrippsiella trochoidea were produced using N-limited medium. The restingcysts were inactivated using hydroxyl radicals based on a strong ionization discharge. The results showed that hy- droxyl radicals could inactivate the resting cysts effectively when the CT value was 0.49 mg · min/L with a contact time of 10 s. Morphological changes were observed using scanning electron microscope which showed that hydroxyl radicals could destroy the thick cyst wall. After entering the cell, hydroxyl radicals may damage the chlorophyll and DNA. Also, the germination rate decreased to zero. In general, the · OH treatment can be applied to inacti- vate the resting cysts of S. trochoidea and provide a new method to kill the harmful algal cysts in ballast water.

关 键 词：羟基自由基 强电离放电 赤潮 休眠性孢囊 锥状斯氏藻 

分 类 号：U698.7[交通运输工程—港口、海岸及近海工程]