化感水稻苗期茎叶组织中萜类物质检测和抑草活性分析  

Determination of terpenoids in the stems and leaves of allelopathic rice seedlings and their weed-suppressive activity

作　　者：李家玉[1] 林志华[1] 李立[1] 杨小燕[1] 张奇[1] 何海斌[1] 

机构地区：[1]福建省农业生态过程与安全监控重点实验室,福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2017年第2期129-134,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金(31370380,31070447);闽台作物特色种质创制与绿色栽培协同创新中心福建2011项目(NO.2015-75)

摘　　要：采用稻稗抑草圈土壤盆栽种植方法,微波萃取和气质联用色谱技术测定苗期强化感水稻PI312777与非化感水稻Lemont的茎、叶中萜种类及总萜、单萜、含氧单萜浓度,并进行比较分析.根据物质分析结果,进一步考察含氧萜类对稗草的抑制能力.结果表明:化感水稻PI312777显著抑制其周围稗草的生长,且其苗期茎叶中总萜、单萜以及含氧单萜含量均高于非化感水稻Lemont.验证结果表明,所选择的7种含氧单萜的混合溶液对稗草的株高和干重的抑制作用随着浓度的增高而升高,且低浓度的含氧单萜混合溶液对常规水稻Ⅱ优航无危害作用,因此化感水稻苗期自然分泌的含氧单萜物质是一种潜在的生态安全除草剂.In order to explore the effect of terpenoids from allelopathic rice on paddy weed,allelopathic rice cultivar PI312777,nonallelopathic rice cultivar Lemont,and barnyardgrass（ Echinochloa crusgalli L.） were mixed cultured by inhibitory circle method in pot experiment with paddy soil,with the distance between rice and barnyardgrass being 12 cm. Contents of total terpenoid,monoterpenoid,and oxygenated monoterpenoid in the leaves and stem of rice seedlings were determined by GC-MS technique with microwave-assisted extraction. The results showed that the allelopathic rice PI312777 effectively suppressed the growth of adjacent barnyardgrass,and the contents of total terpenoids,monoterpenoids,and oxygenated monoterpenoids were higher in the leaves and stems of rice PI312777 than those in Lemont. Inhibitory effect of mixture of the target oxygenated monoterpenoids enhanced with the increased concentrations of the mixed solutions,while without inhibitory effect from conventional rice cultivar,Ⅱ Youhang,at low concentration. In conclusion,oxygenated monoterpenoids potentially could be used as an ecological herbicide.

关 键 词：化感水稻 含氧单萜 GC-MS 抑草圈 抑草活性 

分 类 号：S181[农业科学—农业基础科学]