苎麻根际土壤水浸提液对拟南芥幼苗生长的化感效应  

The Allelopathy of Ramie Rhizosphere Soil Water Extract on Arabidopsis Thaliana Seedling Growth

作　　者：刘楠楠[1] 杨瑞芳[1] 李林林[1] 白玉超[1] 李雪玲[1] 崔国贤[1,2] 

机构地区：[1]湖南农业大学苎麻研究所,长沙410128 [2]湖南农业大学,长沙410128

出　　处：《中国麻业科学》2017年第2期64-68,共5页Plant Fiber Sciences in China

基　　金：国家麻类产业技术体系(CARS-19-E20);国家自然科学基金(31471543);湖南省研究生科研创新(CX2015B232);中国农业科学院科技创新工程(ASTIP-IBFC07)

摘　　要：通过种子萌发实验研究了不同浓度(0、1、2、10 mL/皿)不同苎麻品种根际土壤水浸液对模式植物拟南芥幼苗生长的化感效应,并运用相对实时定量PCR(QPCR)技术从基因水平探讨了不同浓度根际土壤水浸提液处理后与拟南芥生长素合成相关基因YUC1(AT4G32540)的表达。结果表明,随着苎麻根际土壤水浸液浓度的升高,拟南芥幼苗在中苎1号根际土壤处理下其根长与对照相比均呈现出抑制效应,在湘苎7号根际土壤处理下其根长与对照相比呈现出低促高抑现象。在中苎1号根际土壤处理下拟南芥幼苗丙二醛(MDA)含量随着苎麻根际土壤水浸提液浓度升高而上升,在湘苎7号根际土壤处理下拟南芥幼苗丙二醛(MDA)含量在低浓度时,与对照相比降低,当浓度升高,其含量呈现出升高趋势。在不同浓度根际土壤水浸提液处理下,拟南芥幼苗的过氧化物歧化酶(SOD)活性均表现为随处理浓度升高,SOD活性下降,且在中苎1号处理下,下降幅度较湘苎7号大。QPCR检测结果表明,2个苎麻品种处理下YUC1其基因的表达量随水浸提液浓度增加均降低,且中苎1号下降幅度较湘苎7号大。可见,随浸提液浓度升高,苎麻对拟南芥生长的化感胁迫性增强。The seed germination experiment is used to study the different concentrations（0, 1 and 2, 10 mL/dish） and the different ramie varieties rhizosphere soil water extract allelopathic effect on the model piani Arabidopsis thaliana seedlings, and relative real - time quantitative PCR （QPCR） technology was applied to probe into the YUC1 （ AT4G32540） gene expression related to auxin biosynthesis after treatment. Results showed that different concentration of water extracts from rhizosphere soil of ramie had certain allelopathy on the Arabidopsis thaliana, and the allelopathy enhances with the increase of concen-tration. The malondialdehyde （MDA） content of Arabidopsis thaliana seedlings rises with the increase in concentration of extract under the rhizosphere ZhongZhu 1 treatment. Compared with control, malondial-dehyde （MDA） content of seedlings reduced in lower concentration and increased in higher concentration of extract under the rhizosphere Xiangzhu 7 treatment. The superoxide dismutase （SOD） activity de-creased with the rising of treatment concentration. QPCR detection results showed that the gene expres-sive quantity of YUCI reduced with the increase of water extract concentration under the processing of two ramie varieties. In conclusion, with the increase of the extract concentration, ramie has an increase Al-lelopathy on Arabidopsis growth.

关 键 词：苎麻 根际土壤水浸提液 拟南芥 化感效应 抗氧化酶 YUC1基因表达量 

分 类 号：S563.1[农业科学—作物学]