Gomparative analysis of DGAT3（diacylglycerol acyltraiisferase 3） gene from different peanut （Arachis hypogaea L.） varieties  

作　　者：Xiaoping Ren Yanli Zheng Xiaojing Zhou Yuning Chen Li Huang Xiangguo Jiang Guobin Xiao Yong Lei Liying Yan Jiaquan Huang Huifang Jiang Boshou Liao 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of PRC, Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan, China [2]Xiangyang Academy of Agricultural Sciences, Xiangyang, China [3]Jiangxi Institute of Red Soil, Jinxian, China [4]College of Agriculture, Hainan University, Haikou, China

出　　处：《Oil Crop Science》2016年第4期40-48,共9页中国油料作物学报(英文版）

摘　　要：Cultivated peanut is one of the primary sources of vegetable oil and protein in developing countries. DG〉A73 family in peanut cotyledons has no membrane-bound regions suggesting that cytosol is one of the sites for triacylglycerol （TAG） biosynthesis in oilseeds. According to functional annotation and classification of 5 cDNA libraries, 12 unigenes were found with relation of peanut DGAT3 in different organs. Three clones of unigenes, OCP- contig168t OCPcontig12101-3 and OCPcontigl2388-1 were selected for sequencing. Full length sequence of DGAT3 was obtained, showing over 98% sequence similarity with peanut DGAT3 gene AY875644 or EU183333. Upon cluster analysis, DGAT3 of 40 culti- vars were divided into 3 types, namely AhDGATS-1, AhDGAT3-2 and AhDGAT3-3. Coding regions are 1023, 1038 and 1026 base pairs which encoded proteins with 340-, 345- and 341-amino acids, respectively. DGAT3-3 might be a novel gene type among the DGAT3 family which provides great help for studying DGAT3 gene evolution in peanut.

关 键 词：peanut （Arachis hypogaea L.） diacylglycerol acyltransferase 3 （DGAT3） phy-logenetic relationship 

分 类 号：S[农业科学]