HPLC法测定复方双花气雾剂中麻黄碱的含量  

Determination of Ephedrine in the Shuanghua Compound Aerosol by HPLC

作　　者：刘华钢[1] 赖茂祥[2] 陈燕军[1] 谢宇梅 

机构地区：[1]广西医科大学,南宁市滨湖路6号530021 [2]广西中医药研究所

出　　处：《广西科学》2002年第3期210-213,共4页Guangxi Sciences

基　　金：广西科技厅攻关项目

摘　　要：为建立一种用高效液相色谱法测定复方双花气雾剂中麻黄碱含量的方法 ,用 C1 8- ODS为固定相 ,甲醇 -水-三乙胺 (2 0 :80 :0 .1,用磷酸调 p H值 3.5～ 4 .5 )为流动相 ,检测波长为 2 5 7nm。结果表明 ,在进样量 2 .0 4～ 10 .2 0μg范围内 ,该方法线性关系良好 (r=0 .9992 ) ,平均回收率为 98.9% ,RSD为 3.6 1% (n=5 )。表明该方法简便、准确、重复性好 ,可作为该制剂含量的测定方法。To establish a method to determine the content of ephedrine in the Shuanghua compound aerosol by HPLC, C18ODS was used as fixed phase,methanol water triethylamine(20:80:0 1,with the pH value ranging from 3 5 to 4 5 adjusted with phosphoric acid ) as mobile phase. The detection wavelength was 257 nm. It is found that the standard curve is linear in the range of 2 04 to 10 20 μg. The correlation coefficient is 0 9992 The average recovery was 98 9%,and RSD was 3 61% (n=5) It is suggested that the method is simple,convenient,and accurate with a good reduplication ,and could be used to detect the content of ephedrine in the Shuanghua compound aerosol.

关 键 词：HPLC法 复方双花气雾剂 麻黄碱 含量测定 中药 

分 类 号：R284[医药卫生—中药学]