江苏淮北地区小麦品种资源籽粒硬度基因等位变异的KASP检测  

Detection of Allelic Variation for Grain Hardness in Huaibei Region of Jiangsu Province by KASP Markers

作　　者：杨子博[1] 顾正中[1] 周羊梅 王安邦[1] 高平中 熊正海 刘畅[1] 蒋学祥[1] 沈业松 

机构地区：[1]江苏徐淮地区淮阴农业科学研究所/淮安市农业生物技术重点实验室,江苏淮安223001

出　　处：《麦类作物学报》2017年第2期153-161,共9页Journal of Triticeae Crops

基　　金：江苏省科技支撑项目(BE2015352-6;BE2013439);淮安市应用研究与科技攻关(农业)计划项目(HAN2014001)

摘　　要：为了解江苏淮北地区小麦品种资源的籽粒硬度概况及硬度基因型分布规律,以74份近年来江苏淮北地区所育品种(系)和38份来自黄淮其他麦区的常用亲本为材料,采用单籽粒谷物硬度测试仪、KASP标记检测技术和基因扩增及测序技术对其SKCS硬度值及硬度基因型进行鉴定。硬度检测结果表明,供试小麦品种(系)硬度变化范围较大,但硬质麦的比例最大,为70.5%。与常用亲本相比,江苏淮北地区育成品种中软质麦比例较高,为34.3%,但在高代品系中软质麦比例下降到20.5%。基因型检测结果表明,在Puroindoline-D1位点,供试品种(系)中共检测到4种基因型,即野生型(Pina-D1a/Pinb-D1a)、Pina-D1b、Pinb-D1b和Pinb-D1p,其频率依次为25.0%、2.7%、67.9%和4.5%。其中,野生型和Pinb-D1p主要分布在江苏淮北地区。不同硬度基因型的硬度值也存在差异,其中以Pina-D1b基因型的硬度值最高,野生型(Pina-D1a/Pinb-D1a)硬度值最低,Pinb-D1b和Pinb-D1p两硬质类型的籽粒硬度没有显著性差异。在Pinb-2位点,供试品种(系)中共检测到25份材料为Pinb-B2b基因型,包含21份硬质麦、2份混合麦和2份软质麦,其平均硬度值为63.8。To clarify the situation of wheat kernel hardness and the distribution of puroindoline genes in wheat cultivars from Huaibei region of Jiangsu province, a total of 112 wheat cultivars （74 wheat cultivars from Huaibei region of Jiangsu province and 38 breeding parents from Yellow-Huai valley wheat region） were used to test the kernel hardness by a Single Kernel Characterization System （SKCS）. The puroindoline genotype detection was performed in tested cultivars with KASP （Kom- petitive Allele Specific PCR） markers and gene sequencing. The results indicated that the range of kernel hardness was much wider in tested cultivars, and hard wheat was a dominate type with the fre-quency of 70. 5%. Compared with breeding parental cultivars, the percentage of soft wheat in bred cul-tivars from Huaibei region of Jiangsu province was higher （34. 3%） , which was decreased to 20. 5 % in advanced lines. Four puroindoline genotypes were detected in tested cultivars, including Pina-Dla/Pinh-Dla ,Pina-Dlh ,Pinh-Dlh ＆nd Pinb-Dlp with the frequency of 25. 0%,2. 7%, 67. 9% and 4. 5%, respectively. Among them,Pina-Dla/Pinb-Dla axiAPinh-Dlp mainly distributed in Huaibei region of Jiangsu province. The kernel hardness showed difference among different puroindoline genotypes, a-mong which, the kernel hardness index o f Pina-Dlb w a s significantly higher than that of other geno-types. V arieties with Pina-Dl a /Pinh-Dla showed the lowest kernel hardness index, but there were not significant difference between Pinb-Dl b axiAPinh-Dlp. In addition, at th e Pinb-2 locus, 2 5 cultivars in-cluding 21 hard wheats, two soft wheats and two mixed wheats w i th Pinb-B2b allele were detected, with the average hardness index of 63. 8.

关 键 词：小麦 籽粒硬度 PUROINDOLINE基因 KASP 

分 类 号：S512.1[农业科学—作物学] S330