外引大麦SSR标记遗传多样性及其与农艺性状的关联分析  

Analysis of Genetic Diversity and Association with Agronomic Traits in Barley(Hordeum vulgare L.)Introduced from Abroad Using SSR Markers

作　　者：赖勇[1,2] 贾建磊[1,2] 王晋民[1] 任龙[3] 吕仲昱 朱惠琴[1] 马辉[1] 杨莉娜[1] 李宗仁[1] 

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]三江源生态与高原农牧业国家重点实验室,青海西宁810016 [3]青海大学现代教育技术中心,青海西宁810016 [4]甘肃隆源农业科学研究所,甘肃兰州730070

出　　处：《麦类作物学报》2017年第2期197-204,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金地区基金项目(31660429)

摘　　要：为了掌握引进种质资源遗传状况,以55份国外大麦品种为材料,利用SSR分子标记分析了其遗传多样性及性状与标记的关联关系。结果表明,54对SSR标记从55份材料中共检测出154个等位基因、167种基因型。基因多样性变化范围为0.103~0.708,平均值为0.448。PIC变化范围为0.098~0.651,平均值为0.382。不同材料间的遗传相似系数(GS)变化范围为0.533~0.940,平均值为0.722。在GS值约为0.682处可将55份材料分为3类,分别包含49、2、4份材料。群体遗传结构分析将供试材料分成3大亚群,各包含9、24、22份材料。基于GLM模型的关联分析,在P<0.01水平下共检测到16对与株高、穗长、穗下茎长、千粒重和全生育期等5个农艺性状相关联的标记,对性状的解释率的变化范围为6.3%~33.1%。其中,5对标记同时与多个农艺性状相关联。In order to understand the genetic information of introduced germplasm resources, 55 barley accessions from abroad were introduced and genetic diversity and association with agronomic trait was analyzed by SSR markers. A total of 154 alleles were detected from these accessions. There were 164 genotypes among these accessions based on 54 SSR markers. The gene diversity ranged from 0. 103 to0. 708,and the PIC ranged from 0. 098 to 0. 651. The genetic similarity（GS） among these accessions was from 0. 533 to 0. 940, with the average of 0. 722. The accessions were divided into three subgroups at the GS level of 0. 682,in which there were 49,2 and 4 materials,respectively. Based on the analysis of population structure, 55 accessions were composed of three subpopulations, in which there were 9, 24 and 22 materials, respectively. There were 16 markers associated with plant height, spike length, length of ear stem, thousand kernel weight, and days to maturity, based on the general line model （GLM）. The rate of explanation on the phenotype with marker ranged from 0. 063 to 0. 331. And five markers were associated with more than one trait.

关 键 词：大麦 SSR标记 遗传多样性 关联分析 

分 类 号：S512.3[农业科学—作物学] S330