基质金属蛋白酶活性在3种肺疾病中的比较  

Comparison of Matrix Metalloproteinase Activity Between Three Lung Diseases

作　　者：蒋媛[1] 张倩[2] 吴雨丹 周晓鹰[1] 

机构地区：[1]常州大学制药与生命科学学院,江苏常州213164 [2]常州市第二人民医院呼吸科,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2017年第2期47-51,共5页Journal of Changzhou University：Natural Science Edition

基　　金：常州科技局研究基金(KYJ1520305);常州大学高层次人才引进基金(ZMF14020066)

摘　　要：探究基质金属蛋白酶(MMPs)在慢性阻塞性肺病(COPD)、哮喘和肺癌患者中的表达和活性的差异。采用明胶酶谱法来检测COPD、哮喘和肺癌患者血清中MMP表达和活性,其中作图和数据分析都是采用Graph Pad Prism software。结果发现,COPD患者表达MMP-9的水平与哮喘患者相比较高(P<0.05),比肺癌患者相比表达水平更高(P<0.000 1),但是MMP-2在3种病人中表达无明显差异。COPD和哮喘患者表达Pro-MMP-9的概率大于肺癌患者。在COPD、哮喘和肺癌患者中,MMPs酶活性表达存在差异。To explore the serum levels of matrix metalloproteinase（ MMPs） in the patients with chronic obstructive pulmonary disease（ COPD）,asthma and lung carcinoma were compared.Gelatin zymography was used to detect the gelatinolytic activity in serum of the patients with COPD,asthma and lung carcinoma; Graph Pad Prism software was used for photography and statistic analysis.The serum levels of MMP-9 in the patients with COPD were significantly higher than asthmatics（ P 〈0.05） and lung carcinoma（ P〈 0.000 1）.However,there were no significant differences observed among these three patient ＇s groups in the expression level of MMP-2 in serums.The probability of Pro-MMP-9 expression in the patients with COPD and asthma appeared more frequently than the patients with lung carcinoma.There are significant serum level differences of MMP-9 expression between patients with COPD,asthma and lung carcinoma.

关 键 词：基质金属蛋白酶 哮喘 慢性阻塞性肺病 肺癌 

分 类 号：R392.7[医药卫生—免疫学]