2种羊巴贝斯虫的核型及系统发育分析  

Karyotyping and Phylogeny of Two Ovine Babesia Species in China

作　　者：许艳起 张浩浩[3,4] 杨强[3,4] 李超昆 牛怡倩 张改平[1,2,5] 马润林[3] 关贵全[4] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]河南省农业科学院动物免疫学重点实验室,河南郑州450002 [3]中国科学院遗传与发育生物学研究所/分子发育生物学国家重点实验室,北京100101 [4]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [5]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业科学》2017年第3期138-142,共5页Journal of Henan Agricultural Sciences

基　　金：家畜疫病病原生物学国家重点实验室开放课题(2015-MDB-05)

摘　　要：为明确引起羊巴贝斯虫病的病原虫莫氏巴贝斯虫临潭株(Babesia motasi Lintan,BLT)及羊巴贝斯虫未定种新疆株(Babesia sp.Xinjiang,BXJ)的核型和亲缘关系,通过DNA大片段脉冲场凝胶电泳(PFGE)和三代高通量测序对2种虫体进行核型分析和系统发育分析。结果表明:2种巴贝斯虫都有4条染色体,但基因组的大小与核型分析不一致,莫氏巴贝斯虫临潭株基因组大小为11.1 Mb,4条染色体大小分别为6.0 Mb、3.0 Mb、1.1 Mb和1.0 Mb;羊巴贝斯虫未定种新疆株基因组大小为7.0 Mb,4条染色体分别为2.4 Mb、2.0 Mb、1.7 Mb和0.9 Mb;羊巴贝斯虫未定种新疆株与牛巴贝斯虫的亲缘性较近,而莫氏巴贝斯虫临潭株与双芽巴贝斯虫的亲缘关系较近。In order to make sure karyotype and genetic relationship of Babesia motasi Lintan（BLT） and Babesia sp. Xinjiang（BXJ）,the karyotype analysis and phylogenetic analysis were performed by pulsed field gel electrophoresis（PFGE） and 3 generation high-throughput sequencing. The results showed that both of them had 4 chromosomes. But the size of the genome was not consistent with karyotype analysis.Genome size of BLT was 11. 1 Mb,and each chromosome size was 6. 0 Mb,3. 0 Mb,1. 1 Mb and 1. 0 Mb.Genome size of BXJ was 7. 0 Mb with 4 chromosomes of 2. 4 Mb,2. 0 Mb,1. 7 Mb and 0. 9 Mb. Besides,phylogeny of the 2 parasites was analyzed on the basis of genomic data sequenced using third generationsequencing technique. Results indicated that BXJ had close genetic relationship with B. bovis while BLT was more related with B. bigemina.

关 键 词：莫氏巴贝斯虫 羊巴贝斯虫未定种 染色体 核型分析 系统发育分析 

分 类 号：S852.69[农业科学—基础兽医学]