ClC-3基因敲除小鼠的构建及鉴定  

Construction and Identification of ClC-3 Knockout Mouse

作　　者：余杰[1] 毛建文[2] 徐彬[1] 

机构地区：[1]广东药科大学生命科学与生物制药学院,广东广州510006 [2]广东药科大学基础学院,广东广州510006

出　　处：《世界复合医学》2017年第1期10-14,共5页World Journal of Complex Medicine

基　　金：国家自然科学基金:ClC-3氯通道蛋白非离子通道途径调控膜皱褶和有丝分裂的研究(31371144);广东省自然科学基金:氯通道阻断剂NPPB抑制术后腹膜粘连的研究(2016A030313741)~~

摘　　要：目的构建并鉴定ClC-3基因敲除小鼠,为研究ClC-3氯通道蛋白的生物学功能提供动物模型。方法将从2015年9月—2016年9月选取的6只赛业生物科技有限公司(广州)构建的ClC-3flox/+小鼠与2只Jackson实验室引进的Ubc-Cre小鼠进行杂交繁殖,得到基因型为ClC-3flox/flox-Cre+及ClC-3flox/+-Cre+的小鼠。取鼠尾DNA,通过PCR法鉴定子代小鼠的基因型。结果子代小鼠基因组DNA结果显示在357 bp与100 bp处均有条带,符合预期结果。结论该研究基于Cre/Loxp系统,利用loxp转基因的ClC-3flox/+小鼠和在全身表达Cre重组酶的Ubc-Cre小鼠,成功构建并鉴定了ClC-3基因敲除小鼠。Objective To construct and identify the ClC-3 knockout mouse and provide the animal model for researching the biology function of ClC-3 chloride channel protein. Methods 6 ClC-3flox/＋ mice constructed by the Saiye biotechnology Co.Ltd（Guangzhou） and 2 Ubc-Cre mice introduced by Jackson laboratory were selected from September 2015 to September 2016 were selected for interbreeding thus obtaining the l C-3flox/flox-Cre ＋ and ClC-3flox/＋-Cre ＋ mice, Results The generation mice gene was identified by PCR method. The DNA results in the general mice gene group showed that there were stripes at 357 bp and 100 bp, meeting the expected results. Conclusion The paper successfully constructs and identifies the ClC-3 knockout mouse by using the loxp transgenosis ClC-3flox/＋ mice and Ubc-Cre mice with cre recombinase in expression of the whole body based on the Cre/Loxp system.

关 键 词：CLC-3 基因敲除 CRE/LOXP系统 

分 类 号：R4[医药卫生—临床医学]