两株西藏白肉灵芝菌株特性初探  

Isolation,Characterization and Anti-cancer Activity of Two Ganoderma leucocontextum Strains

作　　者：胡惠萍[1] 刘远超[1] 莫伟鹏 黄龙花[1] 张一帆[1] 李泰辉[1] 陈蓉 谢意珍[1,2] 

机构地区：[1]广东省微生物研究所,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510070 [2]广东粤微食用菌技术有限公司,广东广州510663 [3]林芝市科技开发交流服务中心,西藏林芝860000

出　　处：《食用菌学报》2017年第1期50-54,共5页Acta Edulis Fungi

基　　金：广东省科技计划项目(2016B020212008);广州市科技计划项目(201604020005)资助

摘　　要：对西藏林芝地区的白肉灵芝(Ganoderma leucocontextum)菌株G-122和G-241进行鉴别分析,并对其栽培性状、有效成分含量和提取物功能等方面进行研究。基于形态学特性和ITS分析,2个菌株均鉴定为白肉灵芝。二者菌丝对峙培养产生明显拮抗线,菌丝体生物量差异显著(G-241高于G-122),子实体颜色不同(G-241和G-122分别为红褐色和黄褐色),G-241子实体单菇重高于G-122;有效成分含量差异也较大,G-122粗多糖含量高而G-241总三萜含量高;菌株G-122、G-241醇提物对乳腺癌MT-1细胞IC_(50)值分别为265.2和225.6μg/mL。实验结果表明菌株G-122和G-241为不同菌株。Two Ganoderma isolates were collected from the wild near Linzhi City,Tibet,and identified as two separate strains of G.leucocontextum(G-122 and G-241)based on mycelial growth antagonism,fruit body morphology and ITS sequence analysis.Fruit body color also differed(G-122 produced yellow-brown pilei compared to the red-brown pilei of G-241),and the average weight of individual fruit bodies and the biological efficiency were both higher in the case of G-241.Ethanolic extracts of G-122 contained higher levels of crude polysaccharide compared to triterpenoids,whereas the reverse was true for G-241.Both G-122 and G-241 extracts inhibited the proliferation of MT-1breast cancer cells with IC_(50) values of 265.2and225.6μg/mL,respectively.

关 键 词：白肉灵芝 三萜 抗肿瘤 多糖 

分 类 号：S567.31[农业科学—中草药栽培]