重型颅脑损伤的动物实验模型制作及其临床意义  

Development of laboratory animal model of serious traumatic brain injury and its clinical significance

作　　者：应奇[1] 鲁守龙[1] 张风林[1] 徐小龙[1] 唐瑛[2] 焦勇[2] 王銓 王晓熙[3] 张晓莉[3] 鲁宁 

机构地区：[1]解放军第四一一医院,上海200081 [2]海军医学研究所实验动物中心 [3]解放军第四一一医院病理科,上海200081 [4]中石化上海研究院信息中心

出　　处：《海军医学杂志》2017年第2期113-115,130,共4页Journal of Navy Medicine

摘　　要：目的探索可复制重度颅脑损伤实验大鼠动物模型的制作。方法采用改良自由落体装置,撞击80例麻醉后实验鼠颅骨穹窿部,砝码质量50~200 g,高度20~50 cm,撞击后观察时间为4 h^12 d,麻醉后取脑,制作652张病理切片,观察脑病理切片与临床脑创伤的相关性。结果确认最佳冲击力为10 000 g/cm,病理改变与临床有显著相关性,对重型创伤性颅脑损伤的诊治有参考价值。运用模拟临床的闭合性减压术与开放性减压术对比,获得有益提示,后者更有利于脑创伤康复。结论通过本实验找到创伤性重型颅脑损伤大鼠最佳致伤冲击力,实验动物与临床有显著相关性,开放减压比闭合减压更有利于脑康复。Objective To establish replicable animal model of serious traumatic brain injury （S- TBI） in rats. Methods An improved free fall apparatus was used to give impacts on the vault of skull of 80 anesthetized rats with a 50-200 g weight from a 20-50 cm height, the rats were observed for 4 hr to 12 days after impact, and brain was collected after anesthesia to make 652 pathological sections. Results The optimum impact force was determined as 10 000 g/cm. There was a marked correlation between pathological changes and clinical manifestation, which had a reference value to the diagnosis and treatment of S- TBI. A comparison between simula-ted clinical closed decompression and open decompression indicated that the latter was more favorable for the rehabilitation of brain trau-ma. Conclusion The optimum impact force was found in rats of severe traumatic brain injury through experiments; experimental ani-mals were significantly correlative to clinical diagnosis and treatment; open decompression was more beneficial to brain rehabilitation.

关 键 词：重型颅脑损伤 致伤冲击力 临床意义 开放性减压 

分 类 号：R651.15[医药卫生—外科学]