四物汤改善血虚小鼠空间学习记忆能力及其机制  

Effects of Siwu Decoction on Memory and Spatial Learning in Mice with Blood Deficiency and Related Mechanisms

作　　者：李玉洁[1] 孙曙光[1] 张明昊[1] 龚曼[1] 李伟[1] 朱艳琴[1] 

机构地区：[1]河南中医药大学基础医学院,郑州450046

出　　处：《医药导报》2017年第4期370-374,共5页Herald of Medicine

基　　金：河南省科技攻关重点项目(112102310145)

摘　　要：目的观察四物汤对血虚小鼠空间学习记忆能力的影响及其机制。方法将小鼠分为5组:正常对照组、模型对照组和四物汤大、中、小剂量组。模型对照组和四物汤大、中、小剂量组采用连续剪尾放血法造模,3个剂量组分别以2.5,5.0,10.0 g·kg^(-1)四物汤水煎液灌胃14 d。5组于造模前、给药7 d后、给药14 d后,检测小鼠外周血红细胞(RBC)、血红蛋白(HGB)及红细胞比容(HCT)的变化,以T迷宫实验检测各时间点小鼠学习记忆能力,以自主活动检测仪检测各时间点小鼠自主活动情况,并于实验结束时检测5组小鼠血清促红细胞生成素(EPO)与海马乙酰胆碱酯酶(ACh E)含量,探讨四物汤改善血虚小鼠学习记忆能力的机制。结果四物汤5.0,10.0 g·kg^(-1)给药7及14 d后对失血性血虚模型小鼠外周血RBC数量、HGB含量及HCT比值降低有明显的恢复和提高作用,四物汤2.5 g·kg^(-1)给药14 d后对失血性血虚模型小鼠外周血RBC数量及HCT比值降低有明显的恢复和提高作用,四物汤2.5,5.0,10.0 g·kg^(-1)对血虚小鼠自主活动次数有提高作用,并能降低血虚小鼠T迷宫实验选择错误次数,升高血清EPO含量,与模型对照组比较差异有统计学意义(P<0.05或P<0.01)。同时海马ACh E含量有降低趋势,但与模型对照组比较差异无统计学意义(P>0.05)。结论四物汤提高血虚小鼠空间学习记忆能力,其机制可能与提高血清EPO水平及调节海马ACh E含量有关。Objective To investigate the effects of Siwu decoction on the learning and memory abilities in mice with blood deficiency and the related mechanism. Methods The mice were randomly divided into five groups ： normal control group, model control group, high-, medium-, and low-dose group of Siwu decoction. Blood deficiency mouse model was established by continuously cuttingtailand bleedingin model control group, high-, medium-, and low-dose group of Siwu decoction. The mice in high-, medium-, and low-dose group were intragastrically administrated with Siwu decoction of 2. 5 , 5. 0 , 10. 0 g＊kg 1 for 14 days, respectively. The erythrocyte counts （ RBC） , hemoglobin （ HGB） , red blood cell hematocrit （ HCT） were detected before modeling, 7 and 14 days after administration. The spatial learning and memory abilities were assessed using the T maze test. The spontaneous activities were assessed using locomotors activity detector. The levels of erythropoietin （ EPO） content in serum and acetylcholinesterase （AChE） in hippocampal tissue were detected by enzyme-linked immunousorbent assay. Results 5. 0 and 10. 0 g·kg^-1 Siwu decoction could increase RBC, HGB, HCT on the 7th and 14th day, 2. 5 g·kg^-1 Siwu decoction could increase RBC, HCT on 14th day, and each dosage of Siwu decoction could significantly increase the spontaneous activities of model mice with blood deficiency on the 7th and 14th day as compared with model control group （ P 〈 0. 05 or P 〈 0. 01）. Compared with model control group, the number of errorsin T Maze test were decreased significantly in high-, medium-, and low-dose groups （ P 〈 0. 01）. And the Siwu decoction had a tendency to reduce the hippocampal AChE levels, but when compared with the model control group there were no statistical differences （ P 〉 0. 05 ） . Conclusion Siwu decoction is benefit formemory and spatial learning in mice with blood deficiency, which may be relate

关 键 词：四物汤 血虚 空间学习记忆能力 T迷宫 

分 类 号：R285.5[医药卫生—中药学] R749[医药卫生—中医学]